Solid-phase radioimmunoassay method for determination of Escherichia coli enterotoxin.

Infection and immunity ·Vol. 19 ·No. 2 ·1978-02-00 ·Pages 347-52

Ceska M, Grossmüller F, Effenberger F

Abstract

The development of a solid-phase radioimmunoassay procedure for the determination of Escherichia coli enterotoxin(s) is described. Radioiodinated E. coli enterotoxin with about three radioiodine atoms per toxin molecule is, by the criterion of electrophoresis, identical to the unlabeled toxin. Goat anti-E. coli-enterotoxin antibody was coupled to polystyrene tubes and served as a solified toxin binder in the reported procedure. Various conditions necessary for the optimization and standardization of the solid-phase method were established. With the help of this technique it was possible to determine E. coli enterotoxin released from a porcine E. coli strain into culture medium.

MeSH Terms

Bacterial Toxins/analysis,immunology Enterotoxins/analysis,immunology Epitopes Escherichia coli/analysis,immunology Hot Temperature Radioimmunoassay/methods,standards

Chemicals

Bacterial Toxins Enterotoxins Epitopes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ceska M

Grossmüller F

Effenberger F

References (16)

16 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.19.2.347-352.1978

Published

1978-02-00

Pages

347-52

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC414088

Subset

External Links

PubMed source DOI: 10.1128/iai.19.2.347-352.1978 PMC Full Text View journal details

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Solid-phase radioimmunoassay method for determination of Escherichia coli enterotoxin.

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