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PMID: 7592890 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Redundant domains contribute to the transcriptional activity of the thyroid transcription factor 1.

The Journal of biological chemistry ·Vol. 270 ·No. 44 ·1995-11-03 ·Pages 26649-56

De Felice M, Damante G, Zannini M, Francis-Lang H, Di Lauro R

Abstract

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing protein implicated in the activation of thyroid-specific gene expression. Here we report that TTF-1 is capable of activating transcription from thyroglobulin and, to a lesser extent, thyroperoxidase gene promoters in nonthyroid cells. Full transcriptional activation of the thyroglobulin promoter by TTF-1 requires the presence of at least two TTF-1 binding sites. TTF-1 activates transcription via two functionally redundant transcriptional activation domains that as suggested by competition experiments, could use a common intermediary factor.

MeSH Terms

Animals Base Sequence Binding Sites Cell Line Gene Expression Regulation HeLa Cells Homeodomain Proteins/chemistry,metabolism Humans Molecular Sequence Data Mutagenesis, Insertional Nuclear Proteins/biosynthesis,chemistry,metabolism Oligodeoxyribonucleotides Promoter Regions, Genetic Rats Recombinant Fusion Proteins/biosynthesis,metabolism Recombinant Proteins/chemistry,metabolism TATA Box Thyroglobulin/biosynthesis,genetics Thyroid Gland/metabolism Thyroid Nuclear Factor 1 Transcription Factors/biosynthesis,chemistry,metabolism Transcription, Genetic Transcriptional Activation Transfection

Chemicals

Homeodomain Proteins NKX2-1 protein, human Nkx2-1 protein, rat Nuclear Proteins Oligodeoxyribonucleotides Recombinant Fusion Proteins Recombinant Proteins Thyroid Nuclear Factor 1 Transcription Factors Thyroglobulin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

De Felice M

Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.

Damante G

Zannini M

Francis-Lang H

Di Lauro R

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

DOI

10.1074/jbc.270.44.26649

Published

1995-11-03

Pages

26649-56

Language

English

Region

United States

NLM ID

2985121R

Subset

External Links

PubMed source DOI: 10.1074/jbc.270.44.26649 View journal details

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Redundant domains contribute to the transcriptional activity of the thyroid transcription factor 1.

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