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PMID: 7601454 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of the human invasion suppressor gene E-cadherin (CDH1).

Genomics ·Vol. 26 ·No. 2 ·1995-03-20 ·Pages 281-9

Berx G, Staes K, van Hengel J, Molemans F, Bussemakers MJ, van Bokhoven A, van Roy F

Abstract

E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. By using recombinant lambda phage, cosmid, and P1 phage clones, we isolated the full-length human E-cadherin gene (CDH1). The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1 was confirmed by FISH analysis. Detailed restriction mapping and partial sequence analysis of the gene allowed us to identify 16 exons and a 65-kb-long intron 2. The intron-exon boundaries are highly conserved in comparison with other "classical cadherins." In intron 1 we identified a 5' high-density CpG island that may be implicated in transcription regulation during embryogenesis and malignancy.

Related Genes
CDH1
MeSH Terms
Amino Acid Sequence Base Sequence Cadherins/genetics Cell Adhesion Chromosome Mapping Chromosomes, Human, Pair 16 Cloning, Molecular Enhancer Elements, Genetic Exons Gene Expression Regulation Genes Humans In Situ Hybridization, Fluorescence Introns Molecular Sequence Data Neoplasm Invasiveness Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Cadherins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Berx G
Section Molecular Cell Biology, University of Ghent, Belgium.
Staes K
van Hengel J
Molemans F
Bussemakers M J
van Bokhoven A
van Roy F
Article Info
Journal
Genomics
Abbr.
Genomics
ISSN
0888-7543
DOI
10.1016/0888-7543(95)80212-5
Published
1995-03-20
Pages
281-9
Language
English
Region
United States
NLM ID
8800135
Subset
IM
Databases
GENBANK
L34784, L34785, L34786, L34787, L34788, L34789, L34790, L34791, L34792, L34793, L34794, L34795, L34796, L34797, L34936, L34937, L36526, Z35402, Z35403, Z35404, Z35405, Z35406, Z35407, Z35408, Z35409, Z35410, Z35411, Z35412, Z35413, Z35414
External Links
PubMed source DOI: 10.1016/0888-7543(95)80212-5 View journal details
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