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PMID: 7601841 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The sigma 54-dependent promoter Ps of the TOL plasmid of Pseudomonas putida requires HU for transcriptional activation in vivo by XylR.

Journal of bacteriology ·Vol. 177 ·No. 13 ·1995-07-00 ·Pages 3758-63

Pérez-Martín J, de Lorenzo V

Abstract

In the presence of toluene and xylenes, the sigma 54-dependent Ps promoter of the TOL (toluene biodegradation) plasmid pWW0 of Pseudomonas putida is activated at a distance by the XylR protein, of the NtrC family of transcriptional regulators. Since contacts between XylR bound to upstream activating sites and the RNA polymerase require the looping out of the intervening DNA segment, the intrinsic curvature, the bendability of the corresponding sequence, and the spatial effects of protein-induced DNA bending have an influence on promoter activity. Unlike other sigma 54-dependent promoters, Ps does not require the structural aid of the integration host factor to assemble a specific promoter geometry required for transcriptional initiation. In vivo analysis of transcriptional activity in various genetic backgrounds suggests, instead, that the looping out of intervening DNA sequences in Ps would result from the exacerbation of a preexisting static bend within the region, assisted by the histone-like protein HU.

MeSH Terms
Bacterial Proteins/genetics,metabolism DNA-Binding Proteins/genetics,metabolism DNA-Directed RNA Polymerases/metabolism Gene Expression Regulation, Bacterial Integration Host Factors Nucleic Acid Conformation Plasmids/genetics Promoter Regions, Genetic/genetics Pseudomonas putida/genetics RNA Polymerase Sigma 54 Sigma Factor/metabolism Toluene/metabolism Transcription Factors Transcription, Genetic Xylenes/metabolism
Chemicals
Bacterial Proteins DNA-Binding Proteins Integration Host Factors Sigma Factor Transcription Factors XylR protein, Pseudomonas Xylenes histone-like protein HU, bacteria integration host factor, Pseudomonas Toluene DNA-Directed RNA Polymerases RNA Polymerase Sigma 54
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pérez-Martín J
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
de Lorenzo V
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.13.3758-3763.1995
Published
1995-07-00
Pages
3758-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177093
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.177.13.3758-3763.1995 PMC Full Text View journal details
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