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PMID: 7615759 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene.

Journal of clinical microbiology ·Vol. 33 ·No. 5 ·1995-05-00 ·Pages 1375-7

Gunzburg ST, Tornieporth NG, Riley LW

Abstract

A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.

MeSH Terms

Bacteriological Techniques/statistics & numerical data Base Sequence DNA Primers/genetics DNA, Bacterial/genetics Diarrhea/microbiology Escherichia coli/classification,genetics,pathogenicity Escherichia coli Infections/microbiology Evaluation Studies as Topic Fimbriae, Bacterial Genes, Bacterial Humans Infant Molecular Sequence Data Polymerase Chain Reaction/methods,statistics & numerical data Sensitivity and Specificity Serotyping

Chemicals

DNA Primers DNA, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gunzburg S T

Department of Medicine, Cornell University Medical College, New York, New York 10021, USA.

Tornieporth N G

Riley L W

References (17)

17 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.33.5.1375-1377.1995

Published

1995-05-00

Pages

1375-7

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC228170

Subset

External Links

PubMed source DOI: 10.1128/jcm.33.5.1375-1377.1995 PMC Full Text View journal details

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Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene.

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