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PMID: 7615764 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains.

Journal of clinical microbiology ·Vol. 33 ·No. 5 ·1995-05-00 ·Pages 1389-91

Roiz MP, Palenque E, Guerrero C, Garcia MJ

Abstract

Restriction fragment length polymorphism (RFLP) was used to study 75 clinical isolates identified as Mycobacterium avium. Two repetitive insertion sequences, IS1311 and IS900, were used as DNA probes. Although less than 25% of isolates showed RFLP patterns with IS900, all strains gave banding patterns with IS1311. M. avium strains isolated from patients with AIDS exhibited marked polymorphism with both probes.

MeSH Terms
AIDS-Related Opportunistic Infections/complications,microbiology Bacterial Typing Techniques Base Sequence DNA Probes/genetics DNA Transposable Elements DNA, Bacterial/genetics Genetic Markers Humans Molecular Sequence Data Mycobacterium avium Complex/classification,genetics Mycobacterium avium-intracellulare Infection/complications,microbiology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid
Chemicals
DNA Probes DNA Transposable Elements DNA, Bacterial Genetic Markers
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Roiz M P
Departamento de Microbiologia, Hospital Universitario 12 de Octubre, Madrid, Spain.
Palenque E
Guerrero C
Garcia M J
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20 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.33.5.1389-1391.1995
Published
1995-05-00
Pages
1389-91
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC228175
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.33.5.1389-1391.1995 PMC Full Text View journal details
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