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PMID: 7629509 Published · ppublish English Journal Article

Adherence of pilus- Opa+ gonococci to epithelial cells in vitro involves heparan sulfate.

The Journal of experimental medicine ·Vol. 182 ·No. 2 ·1995-08-01 ·Pages 511-7

Chen T, Belland RJ, Wilson J, Swanson J

Abstract

Neisseria gonorrhoeae attaches to host epithelial cells via pili and opacity-associated (Opa) outer membrane proteins. Pilus- gonococci (Gc) of strain MS11 adhere to both human and nonhuman cells, but only when particular Opa proteins are expressed; OpaA+ variants adhere best, OpaC+ variants are next best, and the seven other Opa+ variants adhere poorly or not at all. The adherence of OpaA+ Gc to Chinese hamster ovary (CHO) cells is inhibited by heparin or heparan sulfate (HS), but not by chondroitin sulfate. OpaA+ Gc do not adhere to CHO cells devoid of HS proteoglycans; low concentrations of heparin restore OpaA+ Gc adherence to these HS-deficient CHO cells and high concentrations inhibit it. 3H-heparin binding to whole Gc parallels their adherence abilities (OpaA+ > OpaC+ > OpaH+ >> Opas B, D, E, F, G, I = Opa- = 0). Opa proteins separated by SDS-PAGE also bind 3H-heparin. These data suggest that adherence of pilus-, Opa+ Gc involves HS-proteoglycan of eukaryotic cells.

Related Genes
opaA
MeSH Terms
Animals Antigens, Bacterial/metabolism Bacterial Adhesion Bacterial Outer Membrane Proteins/metabolism CHO Cells Cricetinae Epithelium/microbiology Fimbriae, Bacterial/physiology Heparin/pharmacology Heparitin Sulfate/metabolism Neisseria gonorrhoeae/pathogenicity Protein Binding Protein Denaturation
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins opacity proteins Heparin Heparitin Sulfate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chen T
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA.
Belland R J
Wilson J
Swanson J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.182.2.511
Published
1995-08-01
Pages
511-7
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192128
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.182.2.511 PMC Full Text View journal details
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