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PMID: 7638191 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phototaxis away from blue light by an Escherichia coli mutant accumulating protoporphyrin IX.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 16 ·1995-08-01 ·Pages 7332-6

Yang H, Inokuchi H, Adler J

Abstract

The hemH gene of Escherichia coli encodes ferrochelatase (EC 4.99.1.1), the enzyme that catalyzes the last step in the production of heme, namely the synthesis of heme from protoporphyrin IX plus Fe2+. The behavioral responses to light were studied in E. coli carrying a hemH mutation. It was shown that the hemH mutant displayed a tumbling response upon illumination and a running response upon removal of the light. The most effect light to induce a tumbling response in the hemH mutant was blue light (396-450 nm). The chemotaxis machinery was needed for the light-induced tumbling response in the hemH mutant. The bacterial defect is an analog of the human inherited disease erythropoietic protoporphyria.

Related Genes
hemH
MeSH Terms
Anaerobiosis Cell Movement/genetics,radiation effects Chemotaxis/genetics,radiation effects Escherichia coli/genetics,physiology,radiation effects Ferrochelatase/genetics,metabolism Genes, Bacterial Heme/biosynthesis Humans Light Mutation Oxygen Porphyria, Erythropoietic/genetics Protoporphyrins/genetics,metabolism
Chemicals
Protoporphyrins Heme protoporphyrin IX Ferrochelatase Oxygen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yang H
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706, USA.
Inokuchi H
Adler J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.16.7332
Published
1995-08-01
Pages
7332-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41333
Subset
IM
Grants
NIAID NIH HHS · AI08746 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.16.7332 PMC Full Text View journal details
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