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PMID: 7641684 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functionality and specific membrane localization of transport GTPases carrying C-terminal membrane anchors of synaptobrevin-like proteins.

The EMBO journal ·Vol. 14 ·No. 15 ·1995-08-01 ·Pages 3645-53

Ossig R, Laufer W, Schmitt HD, Gallwitz D

Abstract

Ras-related guanine nucleotide-binding proteins of the Ypt/Rab family fulfill a pivotal role in vesicular protein transport both in yeast and in mammalian cells. Proper functioning of these proteins involves their cycling between a GTP- and a GDP-bound state as well as their reversible association with specific membranes. Here we show that the yeast Ypt1 and Sec4 proteins, essential components of the vesicular transport machinery, allow unimpaired vesicular transport when permanently fixed to membranes by membrane-spanning domains replacing their two C-terminal cysteine residues. Membrane detachment of the GTPases therefore is not obligatory for transport vesicle docking to or fusion with an acceptor membrane. It was also found that the membrane anchors derived from different synaptobrevin-related proteins have targeting information and direct the chimeric GTPases to different cellular compartments, presumably from the endoplasmic reticulum via the secretory pathway.

Related Genes
SEC4 YPT1
MeSH Terms
Amino Acid Sequence Biological Transport Cell Membrane/metabolism Endoplasmic Reticulum/metabolism Fungal Proteins/chemistry,genetics,metabolism GTP Phosphohydrolases/chemistry,genetics,metabolism GTP-Binding Proteins/chemistry,genetics,metabolism Glycoside Hydrolases/metabolism Glycosylation Golgi Apparatus/metabolism Membrane Proteins/chemistry Molecular Sequence Data Nerve Tissue Proteins/chemistry R-SNARE Proteins Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/enzymology,metabolism Saccharomyces cerevisiae Proteins beta-Fructofuranosidase rab GTP-Binding Proteins
Chemicals
Fungal Proteins Membrane Proteins Nerve Tissue Proteins R-SNARE Proteins Recombinant Fusion Proteins SNC1 protein, S cerevisiae SNC2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Glycoside Hydrolases beta-Fructofuranosidase GTP Phosphohydrolases GTP-Binding Proteins YPT1 protein, S cerevisiae SEC4 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ossig R
Department of Molecular Genetics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Laufer W
Schmitt H D
Gallwitz D
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41 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-08-01
Pages
3645-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394439
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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