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PMID: 7644519 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Proofreading-defective DNA polymerase II increases adaptive mutation in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 17 ·1995-08-15 ·Pages 7951-5

Foster PL, Gudmundsson G, Trimarchi JM, Cai H, Goodman MF

Abstract

The role of Escherichia coli DNA polymerase (Pol) II in producing or avoiding mutations was investigated by replacing the chromosomal Pol II gene (polB+) by a gene encoding an exonuclease-deficient mutant Pol II (polBex1). The polBex1 allele increased adaptive mutations on an episome in nondividing cells under lactose selection. The presence of a Pol III antimutator allele (dnaE915) reduced adaptive mutations in both polB+ cells and cells deleted for polB (polB delta 1) to below the wild-type level, suggesting that both Pol II and Pol III are synthesizing episomal DNA in nondividing cells but that in wild-type cells Pol III generates the adaptive mutations. The adaptive mutations were mainly -1 frame-shifts occurring in short homopolymeric runs and were similar in wild-type, polB delta 1, and polBex1 strains. Mutations produced by both Pol III and Pol II ex1 were corrected by the mutHLS mismatch repair system.

Related Genes
dnaE915 polB polB&Dgr 1 polBex1
MeSH Terms
Acclimatization Alleles Base Sequence Chromosomes, Bacterial DNA Polymerase II/metabolism DNA Polymerase III/metabolism DNA Primers Escherichia coli/enzymology,genetics Exodeoxyribonuclease V Exodeoxyribonucleases/genetics Genes, Bacterial Genotype Kinetics Molecular Sequence Data Mutagenesis Mutation Polymerase Chain Reaction
Chemicals
DNA Primers DNA Polymerase II DNA Polymerase III Exodeoxyribonucleases Exodeoxyribonuclease V
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Foster P L
Department of Environmental Health, Boston University School of Public Health, MA 02118-2394, USA.
Gudmundsson G
Trimarchi J M
Cai H
Goodman M F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.17.7951
Published
1995-08-15
Pages
7951-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41264
Subset
IM
Grants
NIA NIH HHS · AG11398 · United States
NIGMS NIH HHS · GM21422 · United States
NIGMS NIH HHS · GM42554 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.17.7951 PMC Full Text View journal details
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