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PMID: 765355 Published · ppublish English Journal Article

Surface immunoglobulin on activated human peripheral blood thymus-derived cells.

The Journal of clinical investigation ·Vol. 57 ·No. 3 ·1976-03-00 ·Pages 762-71

Whiteside TL, Rabin BS

Abstract

Human peripheral blood lymphocytes grown in vitro were stimulated with nonspecific mitogens and in mixed lymphocyte culture. The presence of IgM and thymus (T) surface markers on large and small lymphocytes was investigated by immunofluorescence and correlated with spontaneous rosette formation. All stimulated large lymphocytes formed spontaneous rosettes and all except pokeweed mitogen (PWM)-stimulated large lymphocytes had IgM and T markers. IgG, IgA, and light chain determinants were only detected on PWM-induced large lymphocytes. Thus, surface markers expressed on activated human lymphocytes may differ for different mitogens. IgM was always present on large cells which had the T markers, and it cannot be used to identify a lymphocyte as a bone marrow-derived (B) cell. Due to the overlap of surface markers, the classification into B and thymus-derived (T) cells ought to be restricted to functional phenomena of antibody-production or cell-mediated immunity.

MeSH Terms
Antigens B-Lymphocytes/immunology Cell Membrane/immunology Cells, Cultured Concanavalin A/pharmacology Fluorescent Antibody Technique Humans Immune Adherence Reaction Immune Sera Immunoglobulin M Immunoglobulins Lectins/pharmacology Lymphocyte Activation Mitogens/pharmacology T-Lymphocytes/immunology
Chemicals
Antigens Immune Sera Immunoglobulin M Immunoglobulins Lectins Mitogens Concanavalin A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Whiteside T L
Rabin B S
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45 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108335
Published
1976-03-00
Pages
762-71
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC436712
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108335 PMC Full Text View journal details
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