Location on the chromosome of Escherichia coli of genes governing purine metabolism. Adenosine deaminase (add), guanosine kinase (gsk) and hypoxanthine phosphoribosyltransferase (hpt).

Molecular & general genetics : MGG ·Vol. 143 ·No. 1 ·1975-12-30 ·Pages 85-91

Jochimsen B, Nygaard P, Vestergaard T

Abstract

Genes coding for enzymes functioning in purine salvage pathways have been located on the chromosome of Escherichia coli. The gene add encoding adenosine deaminase was located by transduction at 31 min, the gene order was established to be man-uidA-add-aroD. A deletion covering man-uidA-add was obtained. The gene gsk encoding guanosine kinase was cotransducible with purE and shown to be located at 13 min. The gene hpt encoding hypoxanthine phosphoribosyltransferase was cotransducible with tonA indicating a location at 3 min. The location of the gene gpt encoding guanine (xanthine) phosphoribosyltransferase in the proA-proB region was confirmed.

MeSH Terms

Adenine Phosphoribosyltransferase Adenosine/metabolism Adenosine Deaminase/biosynthesis Chromosome Deletion Chromosome Mapping Chromosomes, Bacterial Conjugation, Genetic Escherichia coli/enzymology,metabolism Genes Guanine Guanosine/metabolism Hypoxanthine Phosphoribosyltransferase/biosynthesis Hypoxanthines/metabolism Inosine/metabolism Mutation Nucleoside Deaminases/biosynthesis Pentosyltransferases/biosynthesis Purines/metabolism Recombination, Genetic Transduction, Genetic Transferases Xanthines

Chemicals

Hypoxanthines Purines Xanthines Guanosine Inosine Guanine Transferases Pentosyltransferases Adenine Phosphoribosyltransferase Hypoxanthine Phosphoribosyltransferase Nucleoside Deaminases Adenosine Deaminase Adenosine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jochimsen B

Nygaard P

Vestergaard T

References (23)

23 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00269424

Published

1975-12-30

Pages

85-91

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00269424

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Location on the chromosome of Escherichia coli of genes governing purine metabolism. Adenosine deaminase (add), guanosine kinase (gsk) and hypoxanthine phosphoribosyltransferase (hpt).

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