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PMID: 765969 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interactions between 30s ribosomal proteins and 50s subunits of Escherichia coli.

Nucleic acids research ·Vol. 3 ·No. 1 ·1976-01-00 ·Pages 1-10

Jenness D, Bruce J, Schaup HW

Abstract

The interaction between ribosomal proteins of the 30s subunit with intact 50s subunits was investigated. Experiments with mixtures of total 30s proteins indicated that several 30s proteins including protein s4 would form a stable complex with 50s subunits. Further work with pure s4 indicates that this protein binds stoichiometrically to the 50s subunits, probably through protein-nucleic acid interaction. The possible significance of this interaction is discussed.

MeSH Terms
Binding Sites Escherichia coli/metabolism Kinetics Macromolecular Substances Protein Binding RNA, Ribosomal/metabolism Ribosomal Proteins/metabolism Ribosomes/metabolism
Chemicals
Macromolecular Substances RNA, Ribosomal Ribosomal Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jenness D
Bruce J
Schaup H W
References (18)
18 references, click to expand
  1. Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli.
    Nature. 1969 Apr 26;222(5191):333-9 PMID: 4181187
  2. Structure and function of ribosomes and their molecular components.
    Cold Spring Harb Symp Quant Biol. 1969;34:49-61 PMID: 4909519
  3. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
    Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449
  4. Reversion of a streptomycin-dependent strain of Escherichia coli.
    Mol Gen Genet. 1970;109(4):356-69 PMID: 4925047
  5. Stoichiometry of the 30S ribosomal proteins of Escherichia coli.
    Biochemistry. 1971 Feb 2;10(3):517-24 PMID: 5543982
  6. Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories.
    Mol Gen Genet. 1971;111(4):327-33 PMID: 4998702
  7. Molecular interactions of ribosomal components. II. Site-specific complex formation between 30S proteins and ribosomal RNA.
    Mol Gen Genet. 1971;112(1):1-8 PMID: 4940422
  8. Chemical modification of the transfer RNA and polyuridylic acid binding sites of Escherichia coli 30 s ribosomal subunits.
    J Mol Biol. 1971 Nov 14;61(3):669-79 PMID: 4943757
  9. Alteration of a 30S ribosomal protein accompanying the ram mutation in Escherichia coli.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2263-7 PMID: 4943794
  10. Ribosomal proteins. XXVI. The number of specific protein binding sites on 16 s and 23 s RNA of Escherichia coli.
    J Mol Biol. 1971 Dec 14;62(2):411-4 PMID: 4944932
  11. Characterization of an RNA "binding site" for a specific ribosomal protein of Escherichia coli.
    Mol Gen Genet. 1972;114(1):1-8 PMID: 4552495
  12. Identification of three 30S proteins contributing to the ribosomal A site.
    Mol Gen Genet. 1972;115(3):234-42 PMID: 4555677
  13. Identification of 50S proteins at the peptidyl-tRNA binding site of Escherichia coli ribosomes.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2799-803 PMID: 4583025
  14. Proteins occurring at, or near, the subunit interface of E. coli ribosomes.
    Mol Gen Genet. 1973 Dec 31;127(4):359-68 PMID: 4594008
  15. Diagonal polyacrylamide-dodecyl sulfate gel electrophoresis for the identification of ribosomal proteins crosslinked with methyl-4-mercaptobutyrimidate.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):3946-50 PMID: 4610565
  16. Localization of Escherichia coli ribosomal proteins S4 and S14 by electron microscopy of antibody-labeled subunits.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4688-92 PMID: 4140507
  17. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  18. Reconstitution of active ribosomes with cross-linked proteins.
    Nat New Biol. 1973 May 2;243(122):15-7 PMID: 17319072
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1976-01-00
Pages
1-10
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342872
Subset
IM
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