Home LiteratureArticle Details
PMID: 7678113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fas involvement in Ca(2+)-independent T cell-mediated cytotoxicity.

The Journal of experimental medicine ·Vol. 177 ·No. 1 ·1993-01-01 ·Pages 195-200

Rouvier E, Luciani MF, Golstein P

Abstract

Mechanisms of T cell-mediated cytotoxicity remain poorly defined at the molecular level. To investigate some of these mechanisms, we used as target cells, on the one hand, thymocytes from lpr and gld mouse mutants, and on the other hand, L1210 cells transfected or not with the apoptosis-inducing Fas molecule. These independent mutant or transfectant-based approaches both led to the conclusion that Fas was involved in the Ca(2+)-independent component of cytotoxicity mediated by at least two sources of T cells, namely nonantigen-specific in vitro activated hybridoma cells, and antigen-specific in vivo raised peritoneal exudate lymphocytes. Thus, in these cases, T cell-mediated cytotoxicity involved transduction via Fas of the target cell death signal.

Related Genes
gld lpr
MeSH Terms
Animals Antigens, Surface/physiology Apoptosis Calcium/physiology Cytotoxicity, Immunologic Mice Mice, Inbred C57BL T-Lymphocytes/immunology fas Receptor
Chemicals
Antigens, Surface fas Receptor Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rouvier E
Centre d'Immunologie Institut National de la Santé et de la Recherche Médicale-Centre National de la Recherche Scientifique de Marseille-Luminy, France.
Luciani M F
Golstein P
References (27)
27 references, click to expand
  1. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation.
    Nature. 1980 Apr 10;284(5756):555-6 PMID: 6245367
  2. DNA fragmentation and cell death is selectively triggered in activated human lymphocytes by Fas antigen engagement.
    Cell Immunol. 1992 Mar;140(1):197-205 PMID: 1371242
  3. Mechanisms of immune lysis. III. Characterization of the nature and kinetics of the cytotoxic T lymphocyte-induced nuclear lesion in the target.
    J Immunol. 1982 May;128(5):2087-94 PMID: 6977583
  4. Hybrids between rat lymphoma and mouse T cells with inducible cytolytic activity.
    Nature. 1982 Jul 8;298(5870):170-2 PMID: 6979720
  5. Characterization of soluble factors that induce the cytolytic activity and the expression of T cell growth factor receptors of a T cell hybrid.
    J Exp Med. 1984 Aug 1;160(2):584-99 PMID: 6236274
  6. T-Lymphocyte-mediated cytolysis as an excitatory process of the target. I. Evidence that the target cell may be the site of Ca2+ action.
    Cell Immunol. 1985 Oct 1;95(1):113-23 PMID: 3928177
  7. YAC-1 MHC class I variants reveal an association between decreased NK sensitivity and increased H-2 expression after interferon treatment or in vivo passage.
    J Immunol. 1985 Dec;135(6):4281-8 PMID: 3905967
  8. Phenotypic, functional, and molecular genetic comparisons of the abnormal lymphoid cells of C3H-lpr/lpr and C3H-gld/gld mice.
    J Immunol. 1986 Jun 1;136(11):4075-84 PMID: 3009614
  9. Cell-associated tumor necrosis factor (TNF) as a killing mechanism of activated cytotoxic macrophages.
    J Immunol. 1987 Feb 1;138(3):957-62 PMID: 3492537
  10. A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: ramifications for the complex physiology of TNF.
    Cell. 1988 Apr 8;53(1):45-53 PMID: 3349526
  11. Tumor necrosis factor and IL-1 associated with plasma membranes of activated human monocytes lyse monokine-sensitive but not monokine-resistant tumor cells whereas viable activated monocytes lyse both.
    J Immunol. 1988 Jul 15;141(2):512-8 PMID: 3260254
  12. A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor.
    J Exp Med. 1989 May 1;169(5):1747-56 PMID: 2469768
  13. Identification and characterization of a membrane-bound cytotoxin of murine cytolytic lymphocytes that is related to tumor necrosis factor/cachectin.
    Proc Natl Acad Sci U S A. 1989 May;86(9):3286-90 PMID: 2654942
  14. Monoclonal antibody-mediated tumor regression by induction of apoptosis.
    Science. 1989 Jul 21;245(4915):301-5 PMID: 2787530
  15. A novel addition to the T cell repertory. Cell surface expression of tumor necrosis factor/cachectin by activated normal human T cells.
    J Exp Med. 1990 Mar 1;171(3):941-6 PMID: 2307938
  16. Monoclonal-antibody-mediated apoptosis in adult T-cell leukaemia.
    Lancet. 1990 Mar 3;335(8688):497-500 PMID: 1689786
  17. Differences defined by bone marrow transplantation suggest that lpr and gld are mutations of genes encoding an interacting pair of molecules.
    J Exp Med. 1990 Nov 1;172(5):1367-75 PMID: 2230649
  18. Anti-Fas monoclonal antibody is cytocidal to human immunodeficiency virus-infected cells without augmenting viral replication.
    Proc Natl Acad Sci U S A. 1990 Dec;87(24):9620-4 PMID: 1702216
  19. Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis.
    Nature. 1992 Mar 26;356(6367):314-7 PMID: 1372394
  20. Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen.
    J Biol Chem. 1992 May 25;267(15):10709-15 PMID: 1375228
  21. DNA fragmentation in targets of CTL: an example of programmed cell death in the immune system.
    Adv Exp Med Biol. 1985;184:493-508 PMID: 2994413
  22. Rejection of ascites tumor allografts. I. Isolation, characterization, and in vitro reactivity of peritoneal lymphoid effector cells from BALB-c mice immune to EL4 leukosis.
    J Exp Med. 1972 Jun 1;135(6):1334-50 PMID: 5025438
  23. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics.
    Br J Cancer. 1972 Aug;26(4):239-57 PMID: 4561027
  24. Limited specific T-cell mediated cytolysis in the absence of extracellular Ca2+.
    Immunology. 1980 Jan;39(1):109-17 PMID: 6769782
  25. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis.
    Cell. 1991 Jul 26;66(2):233-43 PMID: 1713127
  26. The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen.
    J Immunol. 1992 Feb 15;148(4):1274-9 PMID: 1371136
  27. Fine specificity of a continuously growing killer cell clone specific for H-Y antigen.
    Eur J Immunol. 1979 Aug;9(8):592-7 PMID: 91527
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.177.1.195
Published
1993-01-01
Pages
195-200
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190860
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.177.1.195 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2