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PMID: 7679512 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ARIA, a protein that stimulates acetylcholine receptor synthesis, also induces tyrosine phosphorylation of a 185-kDa muscle transmembrane protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 4 ·1993-02-15 ·Pages 1624-8

Corfas G, Falls DL, Fischbach GD

Abstract

Motoneurons promote the accumulation of acetylcholine receptors (AChRs) at developing neuromuscular synapses. The AChR-inducing activity protein ARIA, which is purified from chicken brain and increases the synthesis of AChRs in chicken myotubes, may play a crucial role in this process. Here we show that ARIA induces the rapid tyrosine phosphorylation of a M(r) 185,000 protein (p185) in muscle cells. Phosphorylation of p185 correlates with AChR induction at each stage of ARIA purification. Moreover, medium conditioned by spinal cord motoneurons stimulates AChR synthesis and p185 phosphorylation. Studies with membrane-impermeant reagents and 125I-labeled ARIA indicate that p185 is a transmembrane ARIA-receptor tyrosine kinase. Our data suggests that muscle AChR synthesis can be regulated through tyrosine phosphorylation.

MeSH Terms
Animals Avian Proteins Brain/metabolism Cell Communication Cell Membrane/metabolism Cells, Cultured Chick Embryo Chromatography, Affinity Membrane Proteins/isolation & purification,metabolism Molecular Weight Motor Neurons/physiology Muscles/drug effects,metabolism Nerve Tissue Proteins/isolation & purification,metabolism Neuregulin-1 Phosphoproteins/isolation & purification,metabolism Phosphorylation Phosphotyrosine Rats Receptors, Cholinergic/biosynthesis,metabolism Spinal Cord/physiology Suramin/pharmacology Tyrosine/analogs & derivatives,metabolism
Chemicals
Avian Proteins Membrane Proteins NRG1 protein, Gallus gallus Nerve Tissue Proteins Neuregulin-1 Nrg1 protein, rat Phosphoproteins Receptors, Cholinergic Phosphotyrosine Tyrosine Suramin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Corfas G
Department of Neurobiology, Harvard Medical School, Boston, MA 02115.
Falls D L
Fischbach G D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.4.1624
Published
1993-02-15
Pages
1624-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45927
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.4.1624 PMC Full Text View journal details
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