Minus-strand DNA is present within murine type C ecotropic retroviruses prior to infection.

Journal of virology ·Vol. 67 ·No. 4 ·1993-04-00 ·Pages 2385-8

Zhu J, Cunningham JM

Abstract

Viral minus-strand DNA has been identified within ecotropic murine retroviruses prior to infection. The abundance of minus-strand DNA is inversely proportional to the distance from the primer binding site, suggesting that viral DNA is synthesized by reverse transcriptase with the genomic RNA as template. These findings demonstrate that replication of the retroviral genome is not initiated by infection and may begin after activation of reverse transcriptase by gag-pol cleavage during virus assembly.

MeSH Terms

Animals DNA, Viral/analysis Leukemia Virus, Murine/chemistry,genetics Mice Polymerase Chain Reaction RNA-Directed DNA Polymerase/metabolism Virion/chemistry

Chemicals

DNA, Viral RNA-Directed DNA Polymerase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Zhu J

Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts.

Cunningham J M

References (16)

16 references, click to expand

Presence of DNA in Rous sarcoma virus.
Nature. 1970 Sep 5;227(5262):1023-5 PMID: 4317809
The properties of Rous sarcoma virus purified by density gradient centrifugation.
Virology. 1961 Feb;13:227-32 PMID: 13696318
The biosynthesis of oncovirus proteins.
Biochim Biophys Acta. 1978 Apr 6;473(3-4):187-239 PMID: 77678
In vitro synthesis of a 9 kbp terminally redundant DNA carrying the infectivity of Moloney murine leukemia virus.
Cell. 1979 Apr;16(4):863-74 PMID: 88264
Moloney murine sarcoma virions synthesize full-genome-length double-stranded DNA in vitro.
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3294-8 PMID: 291003
Two species of full-length cDNA are synthesized in high yield by melittin-treated avian retrovirus particles.
Proc Natl Acad Sci U S A. 1980 Feb;77(2):847-51 PMID: 6153806
Form and function of retroviral proviruses.
Science. 1982 May 21;216(4548):812-20 PMID: 6177038
DNA methylation affecting the expression of murine leukemia proviruses.
J Virol. 1982 Oct;44(1):144-57 PMID: 6183444
A deletion mutation in the 5' part of the pol gene of Moloney murine leukemia virus blocks proteolytic processing of the gag and pol polyproteins.
J Virol. 1985 Mar;53(3):899-907 PMID: 3882995
Reverse transcription of mRNA by Thermus aquaticus DNA polymerase.
Nucleic Acids Res. 1989 Oct 25;17(20):8387-8 PMID: 2478963
Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase.
Gene. 1990 Apr 16;88(2):293-6 PMID: 2347496
Amplification, detection, and automated sequencing of gibbon interleukin-2 mRNA by Thermus aquaticus DNA polymerase reverse transcription and polymerase chain reaction.
Anal Biochem. 1990 Nov 1;190(2):292-6 PMID: 2291473
Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase.
Biochemistry. 1991 Aug 6;30(31):7661-6 PMID: 1714296
The human cationic amino acid transporter (ATRC1): physical and genetic mapping to 13q12-q14.
Genomics. 1992 Mar;12(3):430-4 PMID: 1348489
Partial reverse transcripts in virions from human immunodeficiency and murine leukemia viruses.
J Virol. 1992 Aug;66(8):4893-900 PMID: 1378513
Measurement of the sequence complexity of cloned Moloney murine leukemia virus 60 to 70S RNA: evidence for a haploid genome.
J Virol. 1974 Sep;14(3):421-9 PMID: 4368895

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.67.4.2385-2388.1993

Published

1993-04-00

Pages

2385-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC240406

Subset

External Links

PubMed source DOI: 10.1128/JVI.67.4.2385-2388.1993 PMC Full Text View journal details

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Minus-strand DNA is present within murine type C ecotropic retroviruses prior to infection.

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