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PMID: 7683420 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evasion of protective immunity by Borrelia burgdorferi by truncation of outer surface protein B.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 9 ·1993-05-01 ·Pages 4092-6

Fikrig E, Tao H, Kantor FS, Barthold SW, Flavell RA

Abstract

We analyzed variability in outer surface protein B (OspB) from Borrelia burgdorferi (Bb), the causative agent of Lyme disease, to determine how Bb escapes immune destruction. We have shown that vaccination with OspB from Bb strain B31 protected mice from infection with Bb B31 but not against Bb N40. The present study demonstrates that Bb N40 spirochetes which evade vaccination immunity to OspB have a truncated form of OspB, due to a TAA stop codon at nucleotide 577. In contrast, Bb N40 spirochetes that express full-length OspB are unable to infect mice immunized with OspB, analogous to our previous studies with Bb B31. Mapping of the OspB antibody response shows that epitopes in the C terminus of OspB are surface-exposed and bind protective monoclonal and polyclonal antibodies. This suggests that the C terminus of OspB is important for eliciting a protective immune response to OspB. Truncation or modification of outer surface proteins that do not bind protective antibody may be a means by which Bb evades host defenses.

MeSH Terms
Amino Acid Sequence Animals Antibodies Antibodies, Monoclonal Antigens, Bacterial Antigens, Surface/biosynthesis,genetics,immunology Bacterial Outer Membrane Proteins/biosynthesis,genetics,immunology Base Sequence Borrelia burgdorferi Group/genetics,immunology Cloning, Molecular Codon/genetics Epitopes/analysis Escherichia coli/genetics Female Fluorescent Antibody Technique Genes, Bacterial Humans Lyme Disease/immunology,prevention & control Mice Mice, Inbred C3H Polymerase Chain Reaction Recombinant Fusion Proteins/immunology Recombinant Proteins/biosynthesis,immunology Restriction Mapping Ticks/microbiology Vaccines, Synthetic/immunology Viral Vaccines/immunology
Chemicals
Antibodies Antibodies, Monoclonal Antigens, Bacterial Antigens, Surface Bacterial Outer Membrane Proteins Codon Epitopes Recombinant Fusion Proteins Recombinant Proteins Vaccines, Synthetic Viral Vaccines OspB protein, Borrelia burgdorferi
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fikrig E
Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06510.
Tao H
Kantor F S
Barthold S W
Flavell R A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.9.4092
Published
1993-05-01
Pages
4092-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46452
Subset
IM
Grants
NIAID NIH HHS · AI30548 · United States
NIAMS NIH HHS · AR07107 · United States
NIAMS NIH HHS · AR40452 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.9.4092 PMC Full Text View journal details
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