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PMID: 7685274 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

p53-catalyzed annealing of complementary single-stranded nucleic acids.

The EMBO journal ·Vol. 12 ·No. 6 ·1993-06-00 ·Pages 2389-96

Oberosler P, Hloch P, Ramsperger U, Stahl H

Abstract

p53 has been reported to inhibit the DNA helicase intrinsic to simian virus 40 large tumor antigen (T antigen). We found that inhibition is not restricted to T antigen, but also affects several other DNA and RNA helicases. Complexing of the helicases by the p53 protein as a possible inactivation mechanism could be excluded. Instead, the anti-helicase activity can be explained by our finding that p53 binds with high affinity to single-stranded nucleic acids and has a strong DNA.DNA and RNA.RNA annealing activity. We could also show that p53 is able to alter the secondary structure of RNA and/or to influence dynamic RNA-RNA interactions. These results, and the fact that the affinity of p53 to RNA is about one order of magnitude higher than to single-stranded DNA, imply an RNA-specific function of p53 in vivo.

MeSH Terms
Animals Baculoviridae Binding Sites Cells, Cultured Cloning, Molecular DNA Helicases/antagonists & inhibitors DNA, Single-Stranded/metabolism,ultrastructure Humans Microscopy, Electron Moths Nucleic Acid Conformation RNA/chemistry,metabolism,ultrastructure RNA Helicases RNA Nucleotidyltransferases/antagonists & inhibitors Tumor Suppressor Protein p53/metabolism,ultrastructure
Chemicals
DNA, Single-Stranded Tumor Suppressor Protein p53 RNA RNA Nucleotidyltransferases DNA Helicases RNA Helicases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Oberosler P
Fakultät für Biologie, Universität Konstanz, Germany.
Hloch P
Ramsperger U
Stahl H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-06-00
Pages
2389-96
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413469
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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