Home LiteratureArticle Details
PMID: 768985 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Receptor interactions in a signalling system: competition between ribose receptor and galactose receptor in the chemotaxis response.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 3 ·1976-03-00 ·Pages 762-6

Strange PG, Koshland DE

Abstract

Galactose and ribose receptors are shown to compete for a common site by comparing the specificities of the purified receptors with the specificity of the whole organism response. Ribose does not bind to the purified galactose receptor but inhibits the in vivo galactose response. A mutant lacking the ribose receptor shows no inhibition of the galactose response even at very high ribose concentrations. The results can be explained by a mechanism involving (a) a ligand-induced conformational change in the receptor which produces a combining site with affinity for the molecule that serves as a first component of the signalling system and (b) a limited number of such first component molecules that cannot bind both receptors simultaneously. The mechanism may have considerable generality for sensory, hormonal, and transport systems.

MeSH Terms
Allosteric Regulation Binding, Competitive Chemotaxis Galactose/metabolism Monosaccharides/metabolism Mutation Receptors, Drug Ribose/metabolism Salmonella typhimurium
Chemicals
Monosaccharides Receptors, Drug Ribose Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Strange P G
Koshland D E
References (24)
24 references, click to expand
  1. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  2. Adenyl cyclase in fat cells. II. Hormone receptors.
    J Biol Chem. 1969 Jul 10;244(13):3477-82 PMID: 4307453
  3. Rotational and translational diffusion in membranes.
    Annu Rev Biophys Bioeng. 1974;3(0):179-201 PMID: 4371655
  4. Chemoreceptors in bacteria.
    Science. 1969 Dec 26;166(3913):1588-97 PMID: 4902679
  5. Role of the galactose binding protein in chemotaxis of Escherichia coli toward galactose.
    Nat New Biol. 1971 Mar 24;230(12):101-4 PMID: 4927373
  6. Rotational diffusion of rhodopsin in the visual receptor membrane.
    Nat New Biol. 1972 Mar 15;236(63):39-43 PMID: 4537062
  7. Membrane receptors.
    Annu Rev Biochem. 1974;43(0):169-214 PMID: 4368906
  8. Properties of mutants in galactose taxis and transport.
    J Bacteriol. 1974 Feb;117(2):517-26 PMID: 4359648
  9. Brownian motion in biological membranes.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):3111-3 PMID: 1059096
  10. The histidine-binding protein J, a histidine transport component, has two different functional sites.
    J Biol Chem. 1974 Nov 10;249(21):6976-83 PMID: 4213931
  11. Data processing by the chemotaxis machinery of Escherichia coli.
    Nature. 1974 Nov 22;252(5481):317-9 PMID: 4610410
  12. Identification of the ribose binding protein as the receptor for ribose chemotaxis in Salmonella typhimurium.
    Biochemistry. 1974 Oct 22;13(22):4473-8 PMID: 4609457
  13. Structurally defective galactose-binding protein isolated from a mutant negative in the -methylgalactoside transport system of Escherichia coli.
    J Biol Chem. 1972 Sep 10;247(17):5414-24 PMID: 4626720
  14. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli.
    J Gen Microbiol. 1973 Jan;74(1):77-91 PMID: 4632978
  15. Common mechanism for repellents and attractants in bacterial chemotaxis.
    Science. 1973 Jul 6;181(4094):60-3 PMID: 4576832
  16. The hisP protein, a known histidine transport component in Salmonella typhimurium, is also an arginine transport component.
    J Bacteriol. 1973 Oct;116(1):107-13 PMID: 4583201
  17. The regulation and properties of the galactose transport system in Escherichia coli K12.
    J Biol Chem. 1974 Jan 25;249(2):553-8 PMID: 4588568
  18. "Decision"-making in bacteria: chemotactic response of Escherichia coli to conflicting stimuli.
    Science. 1974 Jun 21;184(4143):1292-4 PMID: 4598187
  19. A ribose binding protein of Salmonella typhimurium.
    Biochem Biophys Res Commun. 1972 Sep 26;48(6):1348-53 PMID: 4562155
  20. Components of histidine transport: histidine-binding proteins and hisP protein.
    Proc Natl Acad Sci U S A. 1970 Aug;66(4):1096-103 PMID: 4920090
  21. Transport of sugars and amino acids in bacteria. I. Purification and specificity of the galactose- and leucine-binding proteins.
    J Biol Chem. 1968 Jun 10;243(11):3116-22 PMID: 4871201
  22. Quantitation of the sensory response in bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):710-3 PMID: 1091931
  23. Chemotaxis in bacteria.
    Annu Rev Biochem. 1975;44:341-56 PMID: 1094913
  24. Transport properties of the galactose-binding protein of Escherichia coli. Occurrence of two conformational states.
    J Biol Chem. 1971 Feb 10;246(3):621-8 PMID: 5542675
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.3.762
Published
1976-03-00
Pages
762-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC335998
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.3.762 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2