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PMID: 7690024 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular analyses of the Salmonella g. . . flagellar antigen complex.

Journal of bacteriology ·Vol. 175 ·No. 17 ·1993-09-00 ·Pages 5359-65

Masten BJ, Joys TM

Abstract

Salmonella flagellar filaments are polymers of a highly antigenic protein, termed flagellin. Eight main subfactors have been identified in the Salmonella phase-1 g. . . series flagellar antigen. To determine the molecular basis for expression of the epitopes by which the g. . . family subfactors are distinguished, 10 members of this series were selected and their fliC (the structural gene for phase-1 flagellin) genes were sequenced. Comparative analyses of the inferred primary structures of these flagellins did not allow the identification of linear epitopes responsible for the antigen subfactors. This suggests that conformational aspects are involved in determining the antigenic specificity in these cases. A phylogenetic analysis of the flagellin sequences showed that members of the g. . . series do not form a single coherent unit.

Related Genes
fliC
MeSH Terms
Amino Acid Sequence Antigens, Bacterial/biosynthesis,genetics,immunology Base Sequence Biological Evolution Cloning, Molecular DNA, Bacterial Epitopes/biosynthesis,genetics,immunology Escherichia coli/genetics,immunology Flagellin/biosynthesis,genetics,immunology Molecular Sequence Data Polymerase Chain Reaction Protein Conformation Salmonella/immunology Sequence Homology, Amino Acid
Chemicals
Antigens, Bacterial DNA, Bacterial Epitopes Flagellin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Masten B J
Department of Microbiology, School of Medicine, Texas Tech University Health Sciences Center, Lubbock 79430.
Joys T M
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31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.17.5359-5365.1993
Published
1993-09-00
Pages
5359-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206590
Subset
IM
Databases
GENBANK
Z15064, Z15065, Z15066, Z15067, Z15068, Z15069, Z15070, Z15071, Z15072, Z15086
Corrections
ErratumIn
PMID: 8169233
External Links
PubMed source DOI: 10.1128/jb.175.17.5359-5365.1993 PMC Full Text View journal details
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