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PMID: 7690748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Elucidation of regulatory elements that control damage induction and competence induction of the Bacillus subtilis SOS system.

Journal of bacteriology ·Vol. 175 ·No. 18 ·1993-09-00 ·Pages 5907-15

Cheo DL, Bayles KW, Yasbin RE

Abstract

A novel consensus sequence (GAAC-N4-GTTC) has been identified within the promoter regions of DNA damage-inducible (din) genes from Bacillus subtilis. This sequence has been proposed to function as an operator site that is required for regulation of the SOS system of B. subtilis. To test this hypothesis, a deletion analysis of the dinA and recA promoter regions was utilized. A single consensus sequence is sufficient and necessary for damage-inducible regulation of the dinA and recA promoters. Deletion of the consensus sequences upstream of these promoters derepressed their expression under uninduced conditions. In addition, this deletion analysis has further defined sequences upstream of the recA promoter that are required for expression of the recA gene in cells that have differentiated to the state of natural competence. Northern (RNA) hybridization and S1 nuclease protection experiments have demonstrated that the damage-inducible and competence-inducible recA-specific transcripts initiate from a single promoter. Mutations within the comA, srfA, and degU loci each completely abolish the competence-inducible expression of the recA gene.

Related Genes
din
MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Base Sequence Cloning, Molecular Consensus Sequence DNA Damage DNA, Bacterial Gene Expression Regulation, Bacterial Molecular Sequence Data Promoter Regions, Genetic RNA, Bacterial/analysis RNA, Messenger/analysis Rec A Recombinases/genetics Regulatory Sequences, Nucleic Acid SOS Response, Genetics Transcription, Genetic
Chemicals
DNA, Bacterial RNA, Bacterial RNA, Messenger Rec A Recombinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cheo D L
Department of Biological Sciences, University of Maryland, Baltimore 21228.
Bayles K W
Yasbin R E
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.18.5907-5915.1993
Published
1993-09-00
Pages
5907-15
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206671
Subset
IM
Grants
NIDCR NIH HHS · DE08506 · United States
NIDCR NIH HHS · DE10547 · United States
External Links
PubMed source DOI: 10.1128/jb.175.18.5907-5915.1993 PMC Full Text View journal details
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