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PMID: 7694369 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Photocrosslinking of 5-iodouracil-substituted RNA and DNA to proteins.

Science (New York, N.Y.) ·Vol. 262 ·No. 5137 ·1993-11-19 ·Pages 1255-7

Willis MC, Hicke BJ, Uhlenbeck OC, Cech TR, Koch TH

Abstract

5-Iodouracil-substituted RNA and DNA were crosslinked regiospecifically to associated proteins in yields of 70 to 94% of bound nucleic acid. Irradiation of the iodouracil chromophore with monochromatic, long-wavelength ultraviolet radiation (325 nanometers) eliminates excitation of other nucleic acid and protein chromophores. The combination of high crosslinking yields, excellent specificity, and elimination of photodamage to other chromophores represents an important advance toward the precise identification of contacts in nucleoprotein complexes.

MeSH Terms
Animals Base Sequence Capsid/chemistry Capsid Proteins DNA/chemistry DNA, Protozoan/chemistry DNA, Viral/chemistry Lasers Molecular Sequence Data Nucleic Acid Conformation Nucleoproteins/chemistry Oxytricha Protein Binding Protozoan Proteins/chemistry RNA/chemistry RNA, Viral/chemistry RNA-Binding Proteins Telomere/chemistry Ultraviolet Rays Uracil/analogs & derivatives,chemistry
Chemicals
Capsid Proteins DNA, Protozoan DNA, Viral Nucleoproteins Protozoan Proteins RNA, Viral RNA-Binding Proteins Uracil RNA DNA 5-iodouracil
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Willis M C
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
Hicke B J
Uhlenbeck O C
Cech T R
Koch T H
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.7694369
Published
1993-11-19
Pages
1255-7
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NIGMS NIH HHS · GM-28039 · United States
NIGMS NIH HHS · GM-36944 · United States
External Links
PubMed source DOI: 10.1126/science.7694369 View journal details
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