Home LiteratureArticle Details
PMID: 7695273 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of the PNT1 pentamidine resistance gene of Saccharomyces cerevisiae.

Antimicrobial agents and chemotherapy ·Vol. 38 ·No. 12 ·1994-12-00 ·Pages 2850-6

Ludewig G, Staben C

Abstract

The Saccharomyces cerevisiae PNT1 gene was isolated and characterized. When present in high copy number in S. cerevisiae, PNT1 confers resistance to the anti-Pneumocystis carinii drug pentamidine. The PNT1 gene encodes a previously uncharacterized polypeptide of 409 amino acids. The predicted gene product is a very basic (pI 9.9) polypeptide with one potential membrane-associated region. PNT1 is located on chromosome XVR of S. cerevisiae. It is transcribed at a very low level. Overexpression of the gene increases resistance to the cytostatic and mitochondrial DNA-damaging effects of pentamidine and related cationic compounds. Disruption of the gene leads to slightly increased levels of susceptibility to pentamidine and some related compounds.

Related Genes
PNT1
MeSH Terms
Amino Acid Sequence Antimycin A/pharmacology Base Sequence Chromosome Mapping Drug Resistance/genetics Genes, Fungal Molecular Sequence Data Pentamidine/pharmacology Saccharomyces cerevisiae/drug effects,genetics
Chemicals
Antimycin A Pentamidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ludewig G
T. H. Morgan School of Biological Sciences, University of Kentucky, Lexington 40506-0225.
Staben C
References (26)
26 references, click to expand
  1. Saturation mutagenesis of a polyadenylation signal reveals a hexanucleotide element essential for mRNA 3' end formation in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):257-61 PMID: 8278376
  2. Evolutionary relationships within the fungi: analyses of nuclear small subunit rRNA sequences.
    Mol Phylogenet Evol. 1992 Sep;1(3):231-41 PMID: 1342940
  3. Pentamidine transport and sensitivity in brucei-group trypanosomes.
    J Protozool. 1976 May;23(2):349-56 PMID: 6797
  4. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  5. Nucleotide sequence of the Saccharomyces cerevisiae ADE3 gene encoding C1-tetrahydrofolate synthase.
    J Biol Chem. 1986 Apr 5;261(10):4629-37 PMID: 3514599
  6. Random-clone strategy for genomic restriction mapping in yeast.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7826-30 PMID: 3463999
  7. Flow cytofluorimetric analysis of drug accumulation by multidrug-resistant Trypanosoma brucei brucei and T. b. rhodesiense.
    Mol Biochem Parasitol. 1987 Nov;26(1-2):183-91 PMID: 3431564
  8. Update on pentamidine for the treatment of Pneumocystis carinii pneumonia.
    Clin Pharm. 1988 Jul;7(7):501-10 PMID: 3046830
  9. Animal models for Pneumocystis carinii pneumonia.
    J Protozool. 1989 Jan-Feb;36(1):41-5 PMID: 2651654
  10. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
    Gene. 1988 Dec 30;74(2):527-34 PMID: 3073106
  11. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  12. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
    Curr Genet. 1989 Dec;16(5-6):339-46 PMID: 2692852
  13. Therapy and prophylaxis of systemic protozoan infections.
    Drugs. 1990 Aug;40(2):176-202 PMID: 2121456
  14. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  15. Pneumocystis carinii--new clinical spectrum?
    N Engl J Med. 1991 Jan 24;324(4):263-5 PMID: 1985248
  16. Guide to yeast genetics and molecular biology.
    Methods Enzymol. 1991;194:1-863 PMID: 2005781
  17. Gene overexpression in studies of Saccharomyces cerevisiae.
    Methods Enzymol. 1991;194:239-51 PMID: 2005790
  18. Prevention and treatment of Pneumocystis carinii pneumonia.
    Annu Rev Med. 1991;42:287-95 PMID: 2035974
  19. Automated assembly of protein blocks for database searching.
    Nucleic Acids Res. 1991 Dec 11;19(23):6565-72 PMID: 1754394
  20. Microculture screening assay for primary in vitro evaluation of drugs against Pneumocystis carinii.
    Antimicrob Agents Chemother. 1991 Oct;35(10):1965-74 PMID: 1759815
  21. Pentamidine sensitivity and resistance in Saccharomyces cerevisiae as a model for pentamidine effects on Pneumocystis carinii.
    J Protozool. 1991 Nov-Dec;38(6):70S-71S PMID: 1818214
  22. Inhibition of topoisomerases from Pneumocystis carinii by aromatic dicationic molecules.
    J Protozool. 1991 Nov-Dec;38(6):78S-81S PMID: 1667935
  23. The fungal nature of Pneumocystis.
    J Med Vet Mycol. 1992;30 Suppl 1:271-8 PMID: 1474452
  24. The challenge of Pneumocystis carinii culture.
    J Eukaryot Microbiol. 1993 Mar-Apr;40(2):188-95 PMID: 8461892
  25. A suppressor gene that enables Saccharomyces cerevisiae to grow without making sphingolipids encodes a protein that resembles an Escherichia coli fatty acyltransferase.
    J Biol Chem. 1993 Oct 15;268(29):22156-63 PMID: 8408076
  26. Effects of pentamidine isethionate on Saccharomyces cerevisiae.
    Antimicrob Agents Chemother. 1994 May;38(5):1123-8 PMID: 8067749
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.38.12.2850
Published
1994-12-00
Pages
2850-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC188296
Subset
IM
Grants
NIGMS NIH HHS · GM46193 · United States
Databases
GENBANK
U15217
External Links
PubMed source DOI: 10.1128/AAC.38.12.2850 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2