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PMID: 7697721 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Jagged: a mammalian ligand that activates Notch1.

Cell ·Vol. 80 ·No. 6 ·1995-03-24 ·Pages 909-17

Lindsell CE, Shawber CJ, Boulter J, Weinmaster G

Abstract

Here we report the isolation of a rat cDNA clone, Jagged, which we show encodes a ligand for vertebrate Notch. Our conclusion is based on three observations. First, sequence analysis reveals substantial homology between Jagged and invertebrate ligands for the LIN-12/Notch proteins. Second, in situ hybridization of rat embryos identifies both distinct and overlapping patterns of gene expression for Jagged with those for Notch1, Notch2, and Notch3. Finally, the biological activity of Jagged was tested using a cell culture assay in which Jagged activates rat Notch1 expressed in myoblasts and prevents muscle cell differentiation. Our data support the hypothesis that Notch-ligand interactions function in maintaining mammalian cells in an undifferentiated state.

MeSH Terms

Amino Acid Sequence Animals Blotting, Northern Calcium-Binding Proteins Cloning, Molecular DNA, Complementary Embryo, Mammalian Embryo, Nonmammalian Female Ganglia, Spinal/embryology,metabolism Gene Expression Gene Library Glutathione Transferase/biosynthesis In Situ Hybridization Intercellular Signaling Peptides and Proteins Invertebrates Jagged-1 Protein L Cells Ligands Membrane Proteins/biosynthesis,chemistry,genetics,metabolism Mice Molecular Sequence Data Pregnancy Rats Receptors, Notch Schwann Cells/metabolism Sequence Homology, Amino Acid Serrate-Jagged Proteins Spinal Cord/embryology,metabolism Transfection

Chemicals

Calcium-Binding Proteins DNA, Complementary Intercellular Signaling Peptides and Proteins Jag1 protein, mouse Jag1 protein, rat Jagged-1 Protein Ligands Membrane Proteins Receptors, Notch Serrate-Jagged Proteins Glutathione Transferase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lindsell C E

Department of Biological Chemistry School of Medicine, University of California, Los Angeles 90024.

Shawber C J

Boulter J

Weinmaster G

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/0092-8674(95)90294-5

Published

1995-03-24

Pages

909-17

Language

English

Region

United States

NLM ID

0413066

Subset

Grants

NINDS NIH HHS · NS31885-01 · United States

Databases

GENBANK

L38483, U61276

External Links

PubMed source DOI: 10.1016/0092-8674(95)90294-5 View journal details

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Jagged: a mammalian ligand that activates Notch1.

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