Comparison of polymerase chain reaction and non-radioactive hybridization techniques for the identification of Mycobacterium avium strains.

Molecular and cellular probes ·Vol. 8 ·No. 6 ·1994-12-00 ·Pages 469-71

Thierry D, Matsiota-Bernard P, Nauciel C, Guesdon JL

Abstract

A polymerase chain reaction able to amplify specifically a 205-base-pair DNA fragment of Mycobacterium avium genome was used and compared to a nonradioactive hybridization assay (AccuProbe) and to conventional biochemical identification. The PCR approach to diagnosis of M. avium infection is a valid diagnostic alternative to the more classical procedures.

MeSH Terms

Base Sequence DNA, Bacterial/blood,isolation & purification Luminescent Measurements Molecular Sequence Data Mycobacterium avium/classification,genetics,isolation & purification Nucleic Acid Hybridization Polymerase Chain Reaction Sensitivity and Specificity

Chemicals

DNA, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Thierry D

Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.

Matsiota-Bernard P

Nauciel C

Guesdon J L

Article Info

Journal

Molecular and cellular probes

Abbr.

Mol Cell Probes

ISSN

0890-8508

DOI

10.1006/mcpr.1994.1067

Published

1994-12-00

Pages

469-71

Language

English

Region

England

NLM ID

8709751

Subset

External Links

PubMed source DOI: 10.1006/mcpr.1994.1067 View journal details

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Comparison of polymerase chain reaction and non-radioactive hybridization techniques for the identification of Mycobacterium avium strains.

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