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PMID: 7710952 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts.

British journal of cancer ·Vol. 71 ·No. 4 ·1995-04-00 ·Pages 840-4

Watson CJ, Miller WR

Abstract

The transcription factor, milk protein binding factor (MPBF/Stat5), is a member of the STAT family of signalling molecules which mediates prolactin signal transduction in lactating mammary gland by binding to GAS (gamma-interferon activation site) DNA elements. We have determined the levels of STAT factors in nuclear extracts from a variety of human breast tissues including carcinoma and normal 'resting' breast by electrophoretic mobility-shift assay. The results show that the level of STAT binding activity is low in normal 'resting' breast and benign lesions while carcinoma samples have significantly higher (P < 0.01) amounts of STAT binding activity. Supershift analysis suggests that Stat1 and possibly other members of the STAT family of signalling factors, including Stat3, are activated in breast cancer tissues.

MeSH Terms
Adenofibroma/metabolism,surgery Base Sequence Binding Sites Breast/pathology Breast Diseases/metabolism,surgery Breast Neoplasms/metabolism,surgery Carcinoma in Situ/metabolism,surgery Cell Nucleus/metabolism DNA-Binding Proteins/isolation & purification,metabolism Female Humans Hyperplasia Milk Proteins Molecular Sequence Data Oligodeoxyribonucleotides STAT5 Transcription Factor Trans-Activators/isolation & purification,metabolism Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins Milk Proteins Oligodeoxyribonucleotides STAT5 Transcription Factor Trans-Activators Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watson C J
Roslin Institute (Edinburgh), Midlothian, UK.
Miller W R
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1995.162
Published
1995-04-00
Pages
840-4
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2033751
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1995.162 PMC Full Text View journal details
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