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PMID: 772153 Published · ppublish English Journal Article

Immunity to the group B streptococci: interaction of serum and macrophages with types Ia, Ib, and Ic.

The Journal of experimental medicine ·Vol. 143 ·No. 5 ·1976-05-01 ·Pages 1186-98

Anthony BF

Abstract

The opsonization and phagocytosis of group B streptococci of types Ia, Ib, and Ic were studied in vitro by measuring the uptake of radioactivity by coverslip cultures of rabbit alevolar macrophages during incubation with radiolabeled, nonviable bacteria which had been exposed to rabbit serum. The uptake of counts per minute was quantitative, reproducible, and reversibly inhibited by cold, indicating that it was largely a measurement of phagocytic ingestion rather than of attachment of bacteria-immunoglobulin complexes to macrophage membranes. Moreover, suspended macrophages killed approximately 90% of viable streptococci in the presence of specific antiserum. The opsonic activity of immune serum was heat stable, and phagocytosis of streptococci was insignificant after incubation with normal serum and antiserum to some heterologous group B streptococci. By absorption studies, it was possible to identify the effect of antibodies to specific bacterial antigens. Phagocytosis of streptococci containing the corresponding antigens was maximal after opsonization with homologous or heterologous sera containing antibody to IaCHO, IbCHO, or Ibc protein. Phagocytosis of all three serotypes was intermediate when opsonization could be attributed to anti-IabcCHO. The opsonization of a specific group B streptococcus is complex and may involve two or more antigen-antibody systems.

MeSH Terms
Animals Antibodies, Bacterial Antigen-Antibody Reactions Antigens, Bacterial Female Immunity, Cellular Macrophages/immunology Male Phagocytosis Rabbits Serotyping Streptococcus agalactiae/immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Anthony B F
References (23)
23 references, click to expand
  1. Identification of sialic acid in polysaccharide antigens in group B Streptococcus.
    Infect Immun. 1976 Jan;13(1):284-8 PMID: 55386
  2. Immunochemistry of purified polysaccharide type antigens of group B streptococcal types Ia, Ib, and Ic.
    Infect Immun. 1975 Apr;11(4):845-52 PMID: 804441
  3. Multiple mouse-protective antibodies directed against group B streptococci. Special reference to antibodies effective against protein antigens.
    J Exp Med. 1975 Jul 1;142(1):165-79 PMID: 1097573
  4. Pulmonary host defenses. II. Interaction of respiratory antibodies with Pseudomonas aeruginosa and alveolar macrophages.
    J Immunol. 1973 Aug;111(2):369-80 PMID: 4197983
  5. Cellular and humoral immune response to group B streptococci.
    J Pediatr. 1973 Dec;83(6):926-32 PMID: 4202162
  6. The isolation and some physiochemical and biologic properties of the type 3 antigen of group B streptococci.
    J Immunol. 1972 Jul;109(1):90-6 PMID: 4556260
  7. Nature of lymphocyte-tumor interaction. A general method for cellular immunoabsorption.
    J Exp Med. 1973 Apr 1;137(4):932-42 PMID: 4632629
  8. Distribution by serological type of group B streptococci isolated from a variety of clinical material over a five-year period (with special reference to neonatal sepsis and meningitis).
    Infect Immun. 1973 Aug;8(2):228-35 PMID: 4725698
  9. Studies on interaction of bacteria, serum factors and polymorphonuclear leukocytes in mothers and newborns.
    Pediatrics. 1969 Jul;44(1):49-57 PMID: 4893959
  10. The dissociation of the attachment and ingestion phases of phagocytosis by macrophages.
    Exp Cell Res. 1967 Apr;46(1):19-28 PMID: 4960890
  11. Serological relationships of type I antigens of group B streptococci.
    J Bacteriol. 1969 Feb;97(2):629-34 PMID: 4975151
  12. Type-specific antigens of group B type Ic streptococci.
    Infect Immun. 1971 Nov;4(5):596-604 PMID: 5005310
  13. Type-specific polysaccharide antigens of group B streptococci.
    J Hyg (Lond). 1966 Jun;64(2):191-203 PMID: 5220562
  14. Type-specific polysaccharide antigens of group B streptococci. II. The chemical basis for serological specificity of the type II HCl antigen.
    J Exp Med. 1967 Mar 1;125(3):381-92 PMID: 6018238
  15. In vitro bactericidal capacity of human polymorphonuclear leukocytes: diminished activity in chronic granulomatous disease of childhood.
    J Clin Invest. 1967 Apr;46(4):668-79 PMID: 6021213
  16. Type classification by Lancefield's precipitin method of human and bovine group-B streptococci isolated in Britain.
    J Pathol Bacteriol. 1955 Jan-Apr;69(1-2):43-50 PMID: 13243170
  17. Differentiation of group A streptococci with a common R antigen into three serological types, with special reference to the bactericidal test.
    J Exp Med. 1957 Oct 1;106(4):525-44 PMID: 13475611
  18. Studies of phagocytosis of group A streptococci by polymorphonuclear leucocytes in vitro.
    J Exp Med. 1960 Mar 1;111:309-22 PMID: 13714578
  19. Accessory plasma factors involved in the bactericidal test for type-specific antibody to group A Streptococci. II. Human plasma cofactor (s) enhancing opsonization of encapsulated organisms.
    J Exp Med. 1963 Jan 1;117:1-17 PMID: 13984363
  20. A SEROLOGICAL DIFFERENTIATION OF HUMAN AND OTHER GROUPS OF HEMOLYTIC STREPTOCOCCI.
    J Exp Med. 1933 Mar 31;57(4):571-95 PMID: 19870148
  21. A SEROLOGICAL DIFFERENTIATION OF SPECIFIC TYPES OF BOVINE HEMOLYTIC STREPTOCOCCI (GROUP B).
    J Exp Med. 1934 Mar 31;59(4):441-58 PMID: 19870257
  22. TWO SEROLOGICAL TYPES OF GROUP B HEMOLYTIC STREPTOCOCCI WITH RELATED, BUT NOT IDENTICAL, TYPE-SPECIFIC SUBSTANCES.
    J Exp Med. 1938 Jan 1;67(1):25-40 PMID: 19870707
  23. BACTERIOSTATIC EFFECT OF HUMAN SERA ON GROUP A STREPTOCOCCI : II. COMPARATIVE BACTERIOSTATIC EFFECT OF NORMAL WHOLE BLOOD FROM DIFFERENT ANIMAL SPECIES IN THE PRESENCE OF HUMAN CONVALESCENT SERA.
    J Exp Med. 1945 Aug 1;82(2):107-18 PMID: 19871486
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.143.5.1186
Published
1976-05-01
Pages
1186-98
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190194
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.143.5.1186 PMC Full Text View journal details
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