Isolation of transducing phage carrying rps T, the structural gene for ribosomal protein S20.

Molecular & general genetics : MGG ·Vol. 144 ·No. 1 ·1976-02-27 ·Pages 115-8

Friesen JD, Parker J, Watson RJ, Fiil NP, Pedersen S

Abstract

Lambda transducing phages carrying segments of the Escherichia coli chromosome in the dapB region have been isolated in their in vivo gene products analyzed by two-dimensional gel electrophoresis. One of these phages, lambdaddapB-2, carries the structural genes for ribosomal protein S20 (rps T) and isoleucyltransfer RNA synthetase (ileS.) The most likely gene order is thr-rpsT-ileS-dapB-pyrA.

MeSH Terms

Bacterial Proteins/biosynthesis Chromosome Mapping Chromosomes, Bacterial Coliphages/isolation & purification,metabolism DNA, Bacterial Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism Genes Ribosomal Proteins/biosynthesis Transduction, Genetic

Chemicals

Bacterial Proteins DNA, Bacterial Ribosomal Proteins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Friesen J D

Parker J

Watson R J

Fiil N P

Pedersen S

References (16)

16 references, click to expand

Evidence for a lambda transducing phage carrying the genes for the beta and beta' subunits of Escherichia coli RNA polymerase.
Mol Gen Genet. 1974;132(3):193-201 PMID: 4607989
Identification of a soluble protein that stimulates peptide bond synthesis.
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4257-60 PMID: 1105576
Protein synthesis.
Annu Rev Biochem. 1973;42:397-438 PMID: 4581230
Late steps in the assembly of 30 S ribosomal proteins in vivo in a spectinomycin-resistant mutant of Escherichia coli.
J Mol Biol. 1975 Aug 15;96(3):443-53 PMID: 126327
New chromosomal location for structural genes of ribosomal proteins.
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2765-9 PMID: 1101266
Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase.
J Bacteriol. 1971 Feb;105(2):527-37 PMID: 5541530
A simple method for making new transducing lines of coliphage lambda.
Mol Gen Genet. 1975;137(2):101-7 PMID: 1186657
The structural gene for ribosomal protein S18 in Escherichia coli. III. Mapping outside the ribosomal protein gene cluster at minute 84 on the genome.
Mol Gen Genet. 1974;133(4):329-33 PMID: 4612339
Genetic analysis of an alteration of ribosomal protein S20 in revertants of an alanyl-tRNA-synthetase mutant of Escherichia coli.
Mol Gen Genet. 1974;134(4):325-32 PMID: 4614080
A third kasugamycin resistance locus, ksgC, affecting ribosomal protein S2 in Escherichia coli K-12.
J Bacteriol. 1975 May;122(2):796-7 PMID: 1092672
Specialized transducing phages for ribosomal protein genes of Escherichia coli.
Proc Natl Acad Sci U S A. 1975 Jan;72(1):6-10 PMID: 1090938
High resolution two-dimensional electrophoresis of proteins.
J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
Purification and properties of chain initiation factor 3 from T4-infected and uninfected Escherichia coli MRE 600. Stimulation of translation of synthetic and natural messengers.
J Biol Chem. 1974 Jun 25;249(12):3797-802 PMID: 4601012
Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449
Linkage map of Escherichia coli strain K-12.
Bacteriol Rev. 1972 Dec;36(4):504-24 PMID: 4568762
Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2743-7 PMID: 1101264

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00277314

Published

1976-02-27

Pages

115-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00277314

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Isolation of transducing phage carrying rps T, the structural gene for ribosomal protein S20.

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