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PMID: 772413 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A yeast mutant defective in the processing of 27S r-RNA precursor.

Molecular & general genetics : MGG ·Vol. 144 ·No. 1 ·1976-02-27 ·Pages 29-37

Andrew C, Hopper AK, Hall BD

Abstract

Among a group of 31 ts- yeast mutants screened electrophoretically for heat-sensitive synthesis of each stable RNA species, only mutant ts351 failed to accumulate 25S RNA at 36 degrees C. Pulse-labeling experiments at 36 degrees C showed that 35S and 27S precursors RNA and mature 18S r-RNA molecules are synthetized by ts351 cells but that 25S and 5.8S RNA species are not made and new 60S ribosomal sub-units are not assembled. The mutant is blocked at a specific point in r-RNA processing: the cutting of 27S to form 25S and 5.8S r-RNA.

MeSH Terms
Electrophoresis, Polyacrylamide Gel Fungal Proteins/biosynthesis Hot Temperature Molecular Weight Mutation Nucleic Acid Precursors/metabolism Phenotype RNA, Ribosomal/analysis,biosynthesis RNA, Transfer/biosynthesis Saccharomyces cerevisiae/metabolism
Chemicals
Fungal Proteins Nucleic Acid Precursors RNA, Ribosomal RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Andrew C
Hopper A K
Hall B D
References (14)
14 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00277300
Published
1976-02-27
Pages
29-37
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00277300
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