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PMID: 7751305 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Site of phosphorylation of SpoIIAA, the anti-anti-sigma factor for sporulation-specific sigma F of Bacillus subtilis.

Journal of bacteriology ·Vol. 177 ·No. 10 ·1995-05-00 ·Pages 2912-3

Najafi SM, Willis AC, Yudkin MD

Abstract

Sigma F is regulated by an anti-sigma factor, SpoIIAB, and an anti-anti-sigma factor, SpoIIAA. SpoIIAB also functions as a phosphokinase which transfers phosphate from ATP to SpoIIAA; this phosphorylation is thought to be involved in the regulatory mechanism. By using [gamma-32P]ATP to phosphorylate SpoIIAA, cleaving the protein proteolytically, and analyzing the one resulting radiolabelled peptide by the Edman degradation procedure, we show that the site of phosphorylation in SpoIIAA is Ser-58.

MeSH Terms

Amino Acid Sequence Bacillus subtilis/metabolism Bacterial Proteins/antagonists & inhibitors,metabolism Molecular Sequence Data Phosphorylation Sequence Analysis Sigma Factor Spores, Bacterial/metabolism Transcription Factors

Chemicals

Bacterial Proteins FliA protein, Bacteria Sigma Factor Transcription Factors spoIIR protein, Bacillus subtilis spore-specific proteins, Bacillus

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Najafi S M

Department of Biochemistry, University of Oxford, United Kingdom.

Willis A C

Yudkin M D

References (10)

10 references, click to expand

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Control of developmental transcription factor sigma F by sporulation regulatory proteins SpoIIAA and SpoIIAB in Bacillus subtilis.
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Establishment of cell type by compartmentalized activation of a transcription factor.
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Amino acid sequences at the two sites on glycogen synthetase phosphorylated by cyclic AMP-dependent protein kinase and their dephosphorylation by protein phosphatase-III.
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Microbiol Rev. 1993 Mar;57(1):1-33 PMID: 8464402
Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase.
Cell. 1993 Aug 27;74(4):735-42 PMID: 8358793
An adenosine nucleotide switch controlling the activity of a cell type-specific transcription factor in B. subtilis.
Cell. 1994 Apr 22;77(2):195-205 PMID: 8168129
Role of interactions between SpoIIAA and SpoIIAB in regulating cell-specific transcription factor sigma F of Bacillus subtilis.
Genes Dev. 1994 Nov 1;8(21):2653-63 PMID: 7958923
SpoIIAB is an anti-sigma factor that binds to and inhibits transcription by regulatory protein sigma F from Bacillus subtilis.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.177.10.2912-2913.1995

Published

1995-05-00

Pages

2912-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC176967

Subset

External Links

PubMed source DOI: 10.1128/jb.177.10.2912-2913.1995 PMC Full Text View journal details

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Site of phosphorylation of SpoIIAA, the anti-anti-sigma factor for sporulation-specific sigma F of Bacillus subtilis.

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