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PMID: 775132 Published · ppublish English Journal Article

Function of gene 49 of bacteriophage T4. I. Isolation and biochemical characterization of very fast-sedimenting DNA.

Journal of virology ·Vol. 18 ·No. 3 ·1976-06-00 ·Pages 992-9

Kemper B, Janz E

Abstract

Very fast-sedimenting DNA was isolated from cells after infection with gene 49 defective phage T4. This DNA appeared membrane bound throughout the time after infection and could be isolated either in the membrane-bound form (M-DNA) or free of membrane (released DNA) depending on the lysis procedure. Released DNA formed complexes of marked stability with sedimentation velocities between 1,400S and 2,100S. These complexes did not seem to contain material other than DNA. This was concluded from the results of RNA, protein, and membrane labeling experiments and density analysis. In addition, these complexes were resistant against treatment with n-butanol, phenol. chloroform-methanol, sodium dodecyl sulfate, Sarkosyl, Pronase, RNase, or lysozyme. The observation that more then 90% of the purified very fast-sedimenting DNA is retrapped by magnesium-Sarkosyl crystals (M-band) suggests that the M-band technique may not be sufficient as a test for DNA-membrane attachment.

MeSH Terms
Cell Membrane/metabolism Centrifugation, Density Gradient Coliphages/analysis,metabolism DNA Viruses DNA, Viral/analysis,isolation & purification,metabolism Escherichia coli/metabolism Genes Mutation
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kemper B
Janz E
References (22)
22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.18.3.992-999.1976
Published
1976-06-00
Pages
992-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354799
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.18.3.992-999.1976 PMC Full Text View journal details
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