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PMID: 7753864 Published · ppublish English Comparative Study Journal Article

Pax-6 and lens-specific transcription of the chicken delta 1-crystallin gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 10 ·1995-05-09 ·Pages 4681-5

Cvekl A, Sax CM, Li X, McDermott JB, Piatigorsky J

Abstract

The abundance of delta-crystallin in the chicken eye lens provides an advantageous marker for tissue-specific gene expression during cellular differentiation. The lens-specific expression of the delta 1-crystallin gene is governed by an enhancer in the third intron, which binds a positive (delta EF2) and negative (delta EF1) factor in its core region. Here we show by DNase I footprinting, electrophoretic mobility-shift assays, and cotransfection experiments with the delta 1-promoter/enhancer fused to the chloramphenicol acetyltransferase reporter gene that the delta 1-crystallin enhancer has two adjacent functional Pax-6 binding sites. We also demonstrate by DNase I footprinting that the delta EF1 site can bind the transcription factor USF, raising the possibility that USF may cooperate with Pax-6 in activation of the chicken delta 1- and alpha A-crystallin genes. These data, coupled with our recent demonstration that Pax-6 activates the alpha A-crystallin gene, suggest that Pax-6 may have been used extensively throughout evolution to recruit and express crystallin genes in the lens.

MeSH Terms
Animals Base Sequence Binding Sites Chick Embryo Chickens Chloramphenicol O-Acetyltransferase/biosynthesis Cloning, Molecular Crystallins/biosynthesis DNA-Binding Proteins/biosynthesis,metabolism Enhancer Elements, Genetic Epithelium/metabolism Eye Proteins Fibroblasts/metabolism Gene Expression Guinea Pigs Homeodomain Proteins Lens, Crystalline/metabolism Mice Molecular Sequence Data Oligodeoxyribonucleotides Organ Specificity PAX6 Transcription Factor Paired Box Transcription Factors Recombinant Proteins/biosynthesis Repressor Proteins Sequence Homology, Nucleic Acid Transcription Factors/metabolism Transcription, Genetic Transfection beta-Galactosidase/biosynthesis
Chemicals
Crystallins DNA-Binding Proteins Eye Proteins Homeodomain Proteins Oligodeoxyribonucleotides PAX6 Transcription Factor Paired Box Transcription Factors Pax6 protein, mouse Recombinant Proteins Repressor Proteins Transcription Factors Chloramphenicol O-Acetyltransferase beta-Galactosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cvekl A
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-2730, USA.
Sax C M
Li X
McDermott J B
Piatigorsky J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.10.4681
Published
1995-05-09
Pages
4681-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42008
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.10.4681 PMC Full Text View journal details
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