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PMID: 7760007 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.

The Journal of experimental medicine ·Vol. 181 ·No. 6 ·1995-06-01 ·Pages 2213-20

Bowen MA, Patel DD, Li X, Modrell B, Malacko AR, Wang WC, Marquardt H, Neubauer M, Pesando JM, Francke U

Abstract

Antibody-blocking studies have demonstrated the role of CD6 in thymocyte-thymic epithelial (TE) cell adhesion. Here we report that CD6 expressed by COS cells mediates adhesion to TE cells and that this interaction is specifically blocked with an anti-CD6 monoclonal antibody (mAb) or with a mAb (J4-81) that recognized a TE cell antigen. We isolated and expressed a cDNA clone encoding this antigen and show that COS cells transfected with this cDNA bind a CD6 immunoglobulin fusion protein (CD6-Rg). This antigen, which we named ALCAM (activated leukocyte-cell adhesion molecule) because of its expression on activated leukocytes, appears to be the human homologue of the chicken neural adhesion molecule BEN/SC-1/DM-GRASP. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. We prepared an ALCAM-Rg fusion protein and showed that it binds to COS cell transfectants expressing CD6, demonstrating that ALCAM is a CD6 ligand. The observations that ALCAM is also expressed by activated leukocytes and that both ALCAM and CD6 are expressed in the brain suggest that ALCAM-CD6 interactions may play a role in the binding of T and B cells to activated leukocytes, as well as in interactions between cells of the nervous system.

MeSH Terms
Activated-Leukocyte Cell Adhesion Molecule Amino Acid Sequence Animals Antigens, CD/metabolism Antigens, Differentiation, T-Lymphocyte/metabolism Cell Adhesion Cell Adhesion Molecules/biosynthesis Cell Line Chlorocebus aethiops Chromosome Banding Chromosome Mapping Chromosomes, Human, Pair 3 Consensus Sequence Epithelial Cells Epithelium/physiology Glycoproteins/biosynthesis,genetics,metabolism Humans Karyotyping Ligands Molecular Sequence Data Recombinant Fusion Proteins/biosynthesis Sequence Homology, Amino Acid T-Lymphocytes/cytology,immunology Thymus Gland/cytology,physiology Transfection Tumor Cells, Cultured
Chemicals
Activated-Leukocyte Cell Adhesion Molecule Antigens, CD Antigens, Differentiation, T-Lymphocyte CD6 antigen Cell Adhesion Molecules Glycoproteins Ligands Recombinant Fusion Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Bowen M A
Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA.
Patel D D
Li X
Modrell B
Malacko A R
Wang W C
Marquardt H
Neubauer M
Pesando J M
Francke U
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.181.6.2213
Published
1995-06-01
Pages
2213-20
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192054
Subset
IM
Grants
NCI NIH HHS · CA-28936 · United States
NHGRI NIH HHS · HG-00298 · United States
Databases
GENBANK
L38608
External Links
PubMed source DOI: 10.1084/jem.181.6.2213 PMC Full Text View journal details
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