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PMID: 7760864 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Specificity of diagnostic PCR amplification for M. avium using the probe pMAV22.

Molecular and cellular probes ·Vol. 9 ·No. 1 ·1995-02-00 ·Pages 71-4

Sritharan V, Iralu JV, Barker RH

Abstract

We characterized the serovar specificity of the probe pMAV22 using ATCC isolates. Primers from this sequence amplified DNA from serovars 1-6, 8-10, and ATCC strain 19075 but did not amplify MAIS serovars 7, 11, 12, 14, 17-20. This confirmed that the M. avium probe pMAV22 is specific for M. avium, not M. intracellulare.

MeSH Terms

Base Sequence DNA Primers Humans Molecular Sequence Data Mycobacterium avium/classification,isolation & purification Mycobacterium avium Complex/classification,isolation & purification Mycobacterium avium-intracellulare Infection/diagnosis,virology Oligonucleotide Probes Plasmids Polymerase Chain Reaction/methods Sensitivity and Specificity Tuberculosis/diagnosis,virology

Chemicals

DNA Primers Oligonucleotide Probes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sritharan V

Center for Biotechnology, Anna University, Madras, India.

Iralu J V

Barker R H

Article Info

Journal

Molecular and cellular probes

Abbr.

Mol Cell Probes

ISSN

0890-8508

DOI

10.1016/s0890-8508(95)91067-0

Published

1995-02-00

Pages

71-4

Language

English

Region

England

NLM ID

8709751

Subset

External Links

PubMed source DOI: 10.1016/s0890-8508(95)91067-0 View journal details

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Specificity of diagnostic PCR amplification for M. avium using the probe pMAV22.

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