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PMID: 7761398 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Bclx regulates the survival of double-positive thymocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 11 ·1995-05-23 ·Pages 4763-7

Ma A, Pena JC, Chang B, Margosian E, Davidson L, Alt FW, Thompson CB

Abstract

The bclx gene has been shown to regulate programmed cell death in vitro. We now show that Bclx expression increases dramatically when T cells differentiate from CD4- CD8- (double negative) thymocytes to CD4+ CD8+ [double positive (DP)] thymocytes. In contrast single-positive (SP) thymocytes express negligible amounts of Bclx protein. This expression pattern contrasts with that of Bcl2, which is present in double-negative thymocytes, down-regulated in DP thymocytes, and reinduced upon maturation to SP thymocytes. Elimination of Bclx by gene targeting dramatically shortens the survival of DP thymocytes but not the survival of SP thymocytes or peripheral SP T cells. These data suggest that the induction of Bclx during thymic maturation plays a critical role in regulating the length of time DP thymocytes survive in the absence of selection.

Related Genes
bclx
MeSH Terms
Animals Antibodies, Monoclonal CD4 Antigens/analysis CD8 Antigens/analysis Cell Death Cell Survival/immunology Cells, Cultured Embryo, Mammalian Gene Expression Mice/immunology Proto-Oncogene Proteins/biosynthesis Proto-Oncogene Proteins c-bcl-2 Recombinant Proteins/biosynthesis,immunology Stem Cells/immunology T-Lymphocyte Subsets/cytology,immunology T-Lymphocytes/cytology,immunology bcl-X Protein
Chemicals
Antibodies, Monoclonal Bcl2l1 protein, mouse CD4 Antigens CD8 Antigens Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Recombinant Proteins bcl-X Protein
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ma A
Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA.
Pena J C
Chang B
Margosian E
Davidson L
Alt F W
Thompson C B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.11.4763
Published
1995-05-23
Pages
4763-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41787
Subset
IM
Grants
NIAID NIH HHS · AI-20047 · United States
NIAID NIH HHS · AI-35294 · United States
NCI NIH HHS · CA-42335 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.11.4763 PMC Full Text View journal details
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