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PMID: 7761430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phospholipase D is present on Golgi-enriched membranes and its activation by ADP ribosylation factor is sensitive to brefeldin A.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 11 ·1995-05-23 ·Pages 4952-6

Ktistakis NT, Brown HA, Sternweis PC, Roth MG

Abstract

ADP ribosylation factor (ARF) is a small guanosine triphosphate (GTP)-binding protein that regulates the binding of coat proteins to membranes and is required for several stages of vesicular transport. ARF also stimulates phospholipase D (PLD) activity, which can alter the lipid content of membranes by conversion of phospholipids into phosphatidic acid. Abundant PLD activity was found in Golgi-enriched membranes from several cell lines. Golgi PLD activity was greatly stimulated by ARF and GTP analogs and this stimulation could be inhibited by brefeldin A (BFA), a drug that blocks binding of ARF to Golgi membranes. Furthermore, in Golgi membranes from BFA-resistant PtK1 cells, basal PLD activity was high and not stimulated by exogenous ARF or GTP analogs. Thus, ARF activates PLD on the Golgi complex, suggesting a possible link between transport events and the underlying architecture of the lipid bilayer.

MeSH Terms
ADP-Ribosylation Factors Animals Brefeldin A CHO Cells Carrier Proteins/metabolism Cell Fractionation Cell Line Cricetinae Cyclopentanes/pharmacology Dogs Dose-Response Relationship, Drug Enzyme Activation GTP-Binding Proteins/metabolism Golgi Apparatus/enzymology,ultrastructure Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology Intracellular Membranes/enzymology,ultrastructure Kinetics Orthomyxoviridae/genetics Phospholipase D/analysis,metabolism Protein Binding Protein Synthesis Inhibitors/pharmacology
Chemicals
Carrier Proteins Cyclopentanes Protein Synthesis Inhibitors Brefeldin A Guanosine 5'-O-(3-Thiotriphosphate) Phospholipase D GTP-Binding Proteins ADP-Ribosylation Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ktistakis N T
Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas 72935-9038, USA.
Brown H A
Sternweis P C
Roth M G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.11.4952
Published
1995-05-23
Pages
4952-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41825
Subset
IM
Grants
NIGMS NIH HHS · GM 15817 · United States
NIGMS NIH HHS · GM 31954 · United States
NIGMS NIH HHS · GM 41050 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.11.4952 PMC Full Text View journal details
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