Functional anaerobic electron transport linked to the reduction of nitrate and fumarate in membranes from Escherichia coli as demonstrated by quenching of atebrin fluorescence.

The Biochemical journal ·Vol. 152 ·No. 3 ·1975-12-00 ·Pages 655-9

Haddock BA, Kendall-Tobias MW

Abstract

Measurements were made of energy-dependent quenching of atebrin fluorescence in membrane particles prepared from Escherichia coli grown anaerobically with glycerol as carbon source in the presence of either nitrate or fumarate. It is concluded that this technique can be used to study the functional organization of the anaerobic proton-translocating electron-transport chains that use nitrate or fumarate as terminal electron acceptor.

MeSH Terms

Anaerobiosis Electron Transport Escherichia coli/metabolism Fluorescence Fumarates/metabolism Glycerol/metabolism Nitrates/metabolism Quinacrine

Chemicals

Fumarates Nitrates Quinacrine Glycerol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Haddock B A

Kendall-Tobias M W

References (25)

25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1520655

Published

1975-12-00

Pages

655-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1172520

Subset

External Links

PubMed source DOI: 10.1042/bj1520655 PMC Full Text View journal details

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Functional anaerobic electron transport linked to the reduction of nitrate and fumarate in membranes from Escherichia coli as demonstrated by quenching of atebrin fluorescence.

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