Restriction/modification in Streptococcus thermophilus: isolation and characterization of a type II restriction endonuclease Sth455I.

Applied microbiology and biotechnology ·Vol. 39 ·No. 2 ·1993-05-00 ·Pages 216-20

Guimont C, Henry P, Linden G

Abstract

Streptococcus thermophilus strain CNRZ 455 produces a type II restriction endonuclease designated Sth455I. This enzyme was isolated from cell extracts by anionic and cationic exchange chromatography. This yielded an enzyme preparation free of non-specific nucleases. The optimal reaction conditions for Sth455I are: MgCl2, 30 mM; pH range, 8-9; incubation temperature, 37-42 degrees C; and a high NaCl concentration, 100-200 mM. The results of single- and double-digestion experiments indicates that Sth455I is an isoschizomer of BstNI and EcoRII showing different sensitivity to methylation. The enzyme exhibits restriction activity on the DNA of three bacteriophages of S. thermophilus and no activity on the phage lytic for strain CNRZ 455. The restriction/modification system associated with this strain is discussed.

MeSH Terms

Bacterial Proteins/isolation & purification,metabolism Base Sequence DNA Restriction-Modification Enzymes DNA, Viral/metabolism Deoxyribonucleases, Type II Site-Specific/isolation & purification,metabolism Molecular Sequence Data Streptococcus/enzymology,genetics Streptococcus Phages/genetics Substrate Specificity

Chemicals

Bacterial Proteins DNA Restriction-Modification Enzymes DNA, Viral CCWGG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Guimont C

Laboratory of Applied Biochemistry-INRA Associated Laboratory, Faculty of Sciences, University of Nancy I, Vandoeuvre-les-Nancy, France.

Henry P

Linden G

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13 references, click to expand

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Article Info

Journal

Applied microbiology and biotechnology

Abbr.

Appl Microbiol Biotechnol

ISSN

0175-7598

DOI

10.1007/BF00228609

Published

1993-05-00

Pages

216-20

Language

English

Region

Germany

NLM ID

8406612

External Links

PubMed source DOI: 10.1007/BF00228609 View journal details

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Restriction/modification in Streptococcus thermophilus: isolation and characterization of a type II restriction endonuclease Sth455I.

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