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PMID: 7764025 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A sucrose-synthase gene of Vicia faba L.: expression pattern in developing seeds in relation to starch synthesis and metabolic regulation.

Planta ·Vol. 191 ·No. 3 ·1993-00-00 ·Pages 394-401

Heim U, Weber H, Bäumlein H, Wobus U

Abstract

Copy-DNA clones encoding a single class of sucrose-synthase (SUCS; EC 2.4.1.13) subunit have been isolated and sequenced from a Vicia faba L. seed cotyledonary library. Southern analyses indicated the existence of only one gene. Transcript levels determined by Northern blot hybridisation steadily increased until the middle of development [25-35 days after flowering (DAF)] and declined thereafter. Sucrose levels approximately paralleled levels of SUCS mRNA. The activity of SUCS increased with decreasing fructose and glucose concentrations and peaked about 10 d later than mRNA levels. In-vitro culture experiments demonstrated that increasing the sucrose concentration leads to increased levels of SUCS mRNA. The SUCS mRNA was also synthesised in seed-coat tissue, but in lower amounts than in cotyledons and with a different developmental profile. The early peak level of SUCS mRNA (20 DAF) in seed coats coincided with the peak in the amount of sucrose and with a peak of transiently synthesised starch.

MeSH Terms

Amino Acid Sequence Base Sequence Blotting, Southern DNA Fabaceae/embryology,enzymology,genetics Gene Expression Glucosyltransferases/biosynthesis,genetics Molecular Sequence Data Plants, Medicinal RNA, Messenger/metabolism Restriction Mapping Seeds/enzymology Starch/biosynthesis Sucrose/metabolism Time Factors

Chemicals

RNA, Messenger Sucrose Starch DNA Glucosyltransferases sucrose synthase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Heim U

Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.

Weber H

Bäumlein H

Wobus U

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Article Info

Journal

Planta

Abbr.

Planta

ISSN

0032-0935

DOI

10.1007/BF00195698

Published

1993-00-00

Pages

394-401

Language

English

Region

Germany

NLM ID

1250576

External Links

PubMed source DOI: 10.1007/BF00195698 View journal details

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A sucrose-synthase gene of Vicia faba L.: expression pattern in developing seeds in relation to starch synthesis and metabolic regulation.

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