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PMID: 7770052 Published · ppublish English Comparative Study Journal Article

Two pathogen-responsive genes in parsley encode a tyrosine-rich hydroxyproline-rich glycoprotein (hrgp) and an anionic peroxidase.

Molecular & general genetics : MGG ·Vol. 247 ·No. 4 ·1995-05-20 ·Pages 444-52

Kawalleck P, Schmelzer E, Hahlbrock K, Somssich IE

Abstract

Two recently isolated cDNAs representing genes that are transcriptionally activated in fungus-infected parsley leaves or elicitor-treated, cultured parsley cells are shown to encode a hydroxyproline-rich glycoprotein (HRGP) and an anionic peroxidase. The deduced HRGP protein is rich in tyrosine residues, a feature also found in other pathogen- and wound-induced plant HRGPs. Expression of the peroxidase gene(s) is induced rapidly upon elicitation and precedes that of the HRGP gene. In situ hybridization experiments demonstrate the presence of HRGP and peroxidase mRNAs in parsley tissue around fungal infection sites. Peroxidase mRNA accumulation is particularly sharply restricted to plant cells directly adjacent to fungal hyphae. These results provide further evidence for an important role of specific cell wall modifications in plant defense.

Related Genes
ELI11 ELI9 HRGP
MeSH Terms
Amino Acid Sequence Base Sequence DNA, Complementary/genetics Gene Expression Regulation, Plant Genes, Plant Glycoproteins/chemistry,genetics In Situ Hybridization Magnoliopsida/genetics Molecular Sequence Data Mycoses/genetics Peroxidases/genetics Plant Diseases/genetics Plant Proteins/genetics Sequence Alignment Sequence Homology, Amino Acid Tyrosine
Chemicals
DNA, Complementary Glycoproteins Plant Proteins extensin protein, plant Tyrosine Peroxidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kawalleck P
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, Germany.
Schmelzer E
Hahlbrock K
Somssich I E
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00293146
Published
1995-05-20
Pages
444-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
L36981, L36982
External Links
PubMed source DOI: 10.1007/BF00293146
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