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PMID: 7772418 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel method for transfer of two-dimensional crystals from the air/water interface to specimen grids. EM sample preparation/lipid-layer crystallization.

Journal of structural biology ·Vol. 114 ·No. 1 ·1995-00-00 ·Pages 60-6

Asturias FJ, Kornberg RD

Abstract

Transfer of two-dimensional (2-D) crystals formed on lipid layers by suspension from a wire loop is described. This method gives better recovery and better preservation of 2-D crystals than attained in the past. The method has been applied to crystals of yeast RNA polymerase II to enable their analysis in the frozen hydrated state.

MeSH Terms

Air Carbon Crystallization Fungal Proteins Lipids Microscopy, Electron/instrumentation,methods Proteins RNA Polymerase II Reproducibility of Results Saccharomyces cerevisiae/enzymology Specimen Handling/methods Water

Chemicals

Fungal Proteins Lipids Proteins Water Carbon RNA Polymerase II

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Asturias F J

Department of Cell Biology, Stanford University School of Medicine, California 94305, USA.

Kornberg R D

Article Info

Journal

Journal of structural biology

Abbr.

J Struct Biol

ISSN

1047-8477

DOI

10.1006/jsbi.1995.1005

Published

1995-00-00

Pages

60-6

Language

English

Region

United States

NLM ID

9011206

Subset

Grants

NIAID NIH HHS · AI21144 · United States

External Links

PubMed source DOI: 10.1006/jsbi.1995.1005 View journal details

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A novel method for transfer of two-dimensional crystals from the air/water interface to specimen grids. EM sample preparation/lipid-layer crystallization.

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