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PMID: 7774587 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamic interaction of the protein translocation systems in the inner and outer membranes of yeast mitochondria.

The EMBO journal ·Vol. 14 ·No. 10 ·1995-05-15 ·Pages 2293-7

Horst M, Hilfiker-Rothenfluh S, Oppliger W, Schatz G

Abstract

Mitochondria contain two distinct protein import systems, one in the outer and the other in the inner membrane. These systems can act independently of one another in submitochondrial fractions of if a protein is transported to the outer membrane or to the intermembrane space. It has been proposed that the two systems associate reversibly when a protein is transported across both membranes, but this hypothesis has remained unproven. In order to address this question, we have checked whether antibodies against a subunit of one system can co-immunoprecipitate subunits of the other system. We find that the two systems associate stably if a matrix-targeted precursor is arrested during import; no association is seen in the absence of a stuck precursor. These experiments provide direct evidence that protein import into the mitochondrial matrix is mediated by the reversible interaction of the two translocation systems.

MeSH Terms
Animals Biological Transport Carrier Proteins/metabolism Cell Compartmentation Electron Transport Complex IV/genetics,metabolism Fungal Proteins/metabolism Intracellular Membranes/metabolism Membrane Proteins/metabolism Mice Mitochondria/metabolism Models, Biological Precipitin Tests Protein Binding Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/metabolism Tetrahydrofolate Dehydrogenase/genetics,metabolism
Chemicals
Carrier Proteins Fungal Proteins Membrane Proteins Recombinant Fusion Proteins Tetrahydrofolate Dehydrogenase Electron Transport Complex IV
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Horst M
Biozentrum, University of Basel, Switzerland.
Hilfiker-Rothenfluh S
Oppliger W
Schatz G
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30 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-05-15
Pages
2293-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398336
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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