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PMID: 7777548 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human immunodeficiency virus 1 reservoir in CD4+ T cells is restricted to certain V beta subsets.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 12 ·1995-06-06 ·Pages 5563-7

Dobrescu D, Kabak S, Mehta K, Suh CH, Asch A, Cameron PU, Hodtsev AS, Posnett DN

Abstract

The human immunodeficiency virus 1 (HIV-1) replicates more efficiently in T-cell lines expressing T-cell receptors derived from certain V beta genes, V beta 12 in particular, suggesting the effects of a superantigen. The targeted V beta 12 subset was not deleted in HIV-1-infected patients. It was therefore possible that it might represent an in vivo viral reservoir. Viral load was assessed by quantitative PCR with gag primers and with an infectivity assay to measure competent virus. It was shown that the tiny V beta 12 subset (1-2% of T cells) often has a higher viral load than other V beta subsets in infected patients. Selective HIV-1 replication in V beta 12 cells was also observed 6-8 days after in vitro infection of peripheral blood lymphocytes from normal, HIV-1 negative donors. Viral replication in targeted V beta subsets may serve to promote a biologically relevant viral reservoir.

MeSH Terms
Adult Base Sequence CD4-Positive T-Lymphocytes/virology DNA Primers DNA, Viral/analysis Genes, gag HIV-1/genetics,isolation & purification,pathogenicity,physiology Humans Molecular Sequence Data Polymerase Chain Reaction Receptors, Antigen, T-Cell, alpha-beta/analysis T-Lymphocyte Subsets/virology Virus Replication
Chemicals
DNA Primers DNA, Viral Receptors, Antigen, T-Cell, alpha-beta
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Dobrescu D
Department of Medicine, Cornell University Medical College, New York, NY 10021, USA.
Kabak S
Mehta K
Suh C H
Asch A
Cameron P U
Hodtsev A S
Posnett D N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.12.5563
Published
1995-06-06
Pages
5563-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41736
Subset
IM
Grants
NIAID NIH HHS · AI 033322 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.12.5563 PMC Full Text View journal details
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