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PMID: 7787083 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of high resolution recordings of motor movement.

Biophysical journal ·Vol. 68 ·No. 4 Suppl ·1995-04-00 ·Pages 230S-239S; discussion 239S-241S

Block SM, Svoboda K

Abstract

The development of in vitro motility assays for motor proteins has been accompanied by a parallel development of advanced optical instrumentation capable of recording motion at the molecular level. Devices now exist that can record displacements to better than 0.1 nm at bandwidths in excess of 10 kHz, and that can place controlled forces up to many pN on single motors. Ultra-high resolution data from experiments are now pouring in. The analysis and subsequent interpretation of experimental records, which are inevitably contaminated with high levels of thermal noise, remain an ongoing challenge. This essay examines selected issues relating to this challenge and discusses some alternative approaches.

MeSH Terms
Actins/physiology Adenosine Triphosphate/metabolism Animals Biophysical Phenomena Biophysics Humans In Vitro Techniques Kinesins/physiology Microtubules/physiology Models, Biological Movement/physiology Muscle Contraction/physiology Myosins/physiology Proteins/chemistry,physiology
Chemicals
Actins Proteins Adenosine Triphosphate Myosins Kinesins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Block S M
Department of Molecular Biology, Princeton University, New Jersey 08544, USA.
Svoboda K
References (28)
28 references, click to expand
  1. Tracking kinesin-driven movements with nanometre-scale precision.
    Nature. 1988 Feb 4;331(6155):450-3 PMID: 3123999
  2. Direct observation of kinesin stepping by optical trapping interferometry.
    Nature. 1993 Oct 21;365(6448):721-7 PMID: 8413650
  3. Bead movement by single kinesin molecules studied with optical tweezers.
    Nature. 1990 Nov 22;348(6299):348-52 PMID: 2174512
  4. Sliding distance of actin filament induced by a myosin crossbridge during one ATP hydrolysis cycle.
    Nature. 1985 Jul 25-31;316(6026):366-9 PMID: 4022127
  5. Fluctuation driven ratchets: Molecular motors.
    Phys Rev Lett. 1994 Mar 14;72(11):1766-1769 PMID: 10055695
  6. Microtubule movement by a biotinated kinesin bound to streptavidin-coated surface.
    J Biol Chem. 1994 Mar 18;269(11):8610-5 PMID: 8132586
  7. Myosin step size: estimates from motility assays and shortening muscle.
    J Muscle Res Cell Motil. 1992 Dec;13(6):590-607 PMID: 1491068
  8. Movement of microtubules by single kinesin molecules.
    Nature. 1989 Nov 9;342(6246):154-8 PMID: 2530455
  9. High-resolution tracking of microtubule motility driven by a single kinesin motor.
    Proc Natl Acad Sci U S A. 1994 May 10;91(10):4584-8 PMID: 8183952
  10. Single myosin molecule mechanics: piconewton forces and nanometre steps.
    Nature. 1994 Mar 10;368(6467):113-9 PMID: 8139653
  11. Recombinant kinesin motor domain binds to beta-tubulin and decorates microtubules with a B surface lattice.
    Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1671-5 PMID: 8446580
  12. Mechanochemical coupling in actomyosin energy transduction studied by in vitro movement assay.
    J Mol Biol. 1990 Nov 5;216(1):49-68 PMID: 2146398
  13. Asymmetric pumping of particles.
    Phys Rev Lett. 1994 Apr 18;72(16):2652-2655 PMID: 10055938
  14. Tubulin protofilaments and kinesin-dependent motility.
    J Cell Biol. 1992 Aug;118(4):865-75 PMID: 1500429
  15. Stepwise motion of an actin filament over a small number of heavy meromyosin molecules is revealed in an in vitro motility assay.
    J Biochem. 1994 Apr;115(4):644-7 PMID: 8089077
  16. Fluctuation analysis of motor protein movement and single enzyme kinetics.
    Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11782-6 PMID: 7991536
  17. Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
    Cell. 1985 Aug;42(1):39-50 PMID: 3926325
  18. Kinesin swivels to permit microtubule movement in any direction.
    Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11653-7 PMID: 8265603
  19. Arrangement of subunits in flagellar microtubules.
    J Cell Sci. 1974 May;14(3):523-49 PMID: 4830832
  20. Forced thermal ratchets.
    Phys Rev Lett. 1993 Sep 6;71(10):1477-1481 PMID: 10054418
  21. Force and velocity measured for single kinesin molecules.
    Cell. 1994 Jun 3;77(5):773-84 PMID: 8205624
  22. Sliding distance between actin and myosin filaments per ATP molecule hydrolysed in skinned muscle fibres.
    Nature. 1991 Jul 25;352(6333):352-4 PMID: 1852212
  23. Nano-manipulation of actomyosin molecular motors in vitro: a new working principle.
    Trends Biochem Sci. 1993 Sep;18(9):319-24 PMID: 8236451
  24. Force of single kinesin molecules measured with optical tweezers.
    Science. 1993 Apr 9;260(5105):232-4 PMID: 8469975
  25. Biological applications of optical forces.
    Annu Rev Biophys Biomol Struct. 1994;23:247-85 PMID: 7919782
  26. The force exerted by a single kinesin molecule against a viscous load.
    Biophys J. 1994 Aug;67(2):766-81 PMID: 7948690
  27. Kinesin follows the microtubule's protofilament axis.
    J Cell Biol. 1993 Jun;121(5):1083-93 PMID: 8099076
  28. Single-molecule analysis of the actomyosin motor using nano-manipulation.
    Biochem Biophys Res Commun. 1994 Mar 15;199(2):1057-63 PMID: 8135779
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
Published
1995-04-00
Pages
230S-239S; discussion 239S-241S
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1281932
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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