Characterization of 2-mum DNA of Saccharomyces cerevisiae by restriction fragment analysis and integration in an Escherichia coli plasmid.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 6 ·1976-06-00 ·Pages 2072-6

Hollenberg CP, Degelmann A, Kustermann-Kuhn B, Royer HD

Abstract

Electrophoretic analysis of EcoRI and HindIII restriction fragments of 2-mum supercoiled DNA of Saccharomyces cerevisiae indicated that this class of DNA is heterogeneous and probably consists of two types of molecules. Integration of the 2-mum yeast DNA in E. coli plasmid pCR1 directly showed that existence of two types of molecules as each of these could be individually inserted into separate bacterial plasmids. The difference between the two types of 2-mum circles is due to an inversion of about 1.6 X 10(6) daltons. The inversion is flanked by a reversed duplicated sequence of 0.45 X 10(6) daltons. Possible implications of this structure are dicussed.

MeSH Terms

Chromosome Mapping DNA Restriction Enzymes DNA, Circular/analysis Electrophoresis, Agar Gel Escherichia coli Extrachromosomal Inheritance Genetic Engineering Molecular Weight Plasmids Recombination, Genetic Saccharomyces cerevisiae/analysis

Chemicals

DNA, Circular DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hollenberg C P

Degelmann A

Kustermann-Kuhn B

Royer H D

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26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.6.2072

Published

1976-06-00

Pages

2072-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430451

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.6.2072 PMC Full Text View journal details

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Characterization of 2-mum DNA of Saccharomyces cerevisiae by restriction fragment analysis and integration in an Escherichia coli plasmid.

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