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PMID: 7789757 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae.

Genetics ·Vol. 139 ·No. 4 ·1995-04-00 ·Pages 1521-32

Johzuka K, Ogawa H

Abstract

A temperature-sensitive mre11-1 mutation of Saccharomyces cerevisiae causes defects in meiotic recombination and DNA repair during vegetative growth at a restrictive temperature. We cloned the MRE11 gene and found that it encodes a 643-amino acid protein with a highly acidic region containing a heptad repeat of Asp at its C-terminus and is located downstream of YMR44 near the RNA1 locus on the right arm of chromosome XIII. Transcripts of the MRE11 gene increased transiently and showed the same kinetics as that of the RAD50 gene during meiosis. In a mre11 disruption mutant (mre11::hisG), meiosis-specific double-strand break (DSB) formation is abolished. A comparison of the properties of mre11::hisG and a rad50 deletion mutant (rad50 delta) indicated that both mutants exhibited similar phenotypes in both meiosis and mitosis. Characterization of two double mutants, mre11::hisG rad50 delta and mre11::hisG rad50S, showed that MRE11 and RAD50 belong to the same epistasis group with respect to meiotic DSB formation and mitotic DNA repair. Using a two-hybrid system, we found that Mre11 interacts with Rad50 and itself in vivo. These results suggest that Mre11 and Rad50 proteins work in a complex in DSB formation and DNA repair during vegetative growth.

Related Genes
MRE11 RAD50 RNA1 YMR44
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Repair/genetics DNA, Recombinant DNA-Binding Proteins Endodeoxyribonucleases Exodeoxyribonucleases Fungal Proteins/genetics,metabolism Meiosis/genetics Molecular Sequence Data Mutation RNA, Messenger/genetics Recombination, Genetic Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Transcription, Genetic
Chemicals
DNA, Recombinant DNA-Binding Proteins Fungal Proteins RAD50 protein, S cerevisiae RNA, Messenger Saccharomyces cerevisiae Proteins Endodeoxyribonucleases Exodeoxyribonucleases MRE11 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Johzuka K
Department of Biology, Faculty of Science, Osaka University, Japan.
Ogawa H
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/139.4.1521
Published
1995-04-00
Pages
1521-32
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206481
Subset
IM
Databases
GENBANK
D11463, U60829
External Links
PubMed source DOI: 10.1093/genetics/139.4.1521 PMC Full Text View journal details
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