Home LiteratureArticle Details
PMID: 7790023 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The survival and turnover of mature and immature CD8 T cells.

Immunology ·Vol. 84 ·No. 4 ·1995-04-00 ·Pages 514-20

McDonagh M, Bell EB

Abstract

The present investigation has examined the phenotype, survival and fate of immature and mature CD8 T cells. CD4- CD8+ 'single positive' thymocytes (a model for recent thymic emigrants) were Thy-1+ CD45RC- RT6- before transfer to normal euthymic recipients, but changed phenotype within 7-10 days to become Thy-1- CD45RC+ RT6(+)--the phenotype of mature resting CD8 T cells. Following transfer to athymic nude recipients CD8 T cells from thoracic duct lymph of allotype-marked rats increased 12-17-fold during the first 2 months. Proliferation occurred in the complete absence of CD4 T cells and the donor CD8 T cells persisted [at 15-18% of peripheral blood lymphocytes (PBL)] for the life of the recipients. When combined with equal numbers of CD4 T cells, however, CD8 T cells occupied only 3-4% of PBL; in these animals CD4 T cells plateaued at 15-16% of PBL. The results suggested that CD8 T cells competed poorly with rapidly dividing CD4 T cells for limited space in a recirculating pool in which total T-cell numbers are homeostatically regulated. Although able to proliferate and self-renew in athymic nude recipients, when transferred to normal euthymic animals donor-derived mature CD8 T cells declined in number with time; their half-life was estimated to be 34 days. Similar studies with purified CD4- CD8+ 'single positive' thymocytes gave a comparable half-life of 37 days. The results indicated that lifespan was not due to an ageing process among CD8 T cells, but was rather a reflection of cell turnover dependent on thymic output.

MeSH Terms
Animals CD4-Positive T-Lymphocytes/immunology CD8-Positive T-Lymphocytes/cytology,immunology,transplantation Cell Communication/immunology Cell Differentiation/immunology Cell Division/immunology Cell Survival/immunology Immunomagnetic Separation Leukocyte Common Antigens/analysis Lymphocyte Transfusion Rats Rats, Inbred Strains Rats, Nude T-Lymphocyte Subsets/immunology
Chemicals
Leukocyte Common Antigens
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McDonagh M
Immunology Research Group, Medical School, University of Manchester, UK.
Bell E B
References (51)
51 references, click to expand
  1. A single stem cell can recolonize an embryonic thymus, producing phenotypically distinct T-cell populations.
    Nature. 1985 Oct 31-Nov 6;317(6040):811-3 PMID: 3877245
  2. In vivo treatment of rats with monoclonal anti-T-cell antibodies. Immunohistochemical and functional analysis in normal rats and in experimental allergic neuritis.
    Scand J Immunol. 1985 Aug;22(2):157-69 PMID: 3898352
  3. Analysis of T-cell subsets in rejection of Kb mutant skin allografts differing at class I MHC.
    Nature. 1986 Aug 28-Sep 3;322(6082):829-31 PMID: 3528862
  4. The stable and permanent expansion of functional T lymphocytes in athymic nude rats after a single injection of mature T cells.
    J Immunol. 1987 Sep 1;139(5):1379-84 PMID: 3305705
  5. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
    Mol Immunol. 1987 Dec;24(12):1281-90 PMID: 2828930
  6. Sequential events in thymocyte differentiation and thymus regeneration revealed by a combination of bromodeoxyuridine DNA labeling and antimitotic drug treatment.
    J Immunol. 1988 May 15;140(10):3315-23 PMID: 3258880
  7. The functionally distinct subpopulations of human CD4+ helper/inducer T lymphocytes defined by anti-CD45R antibodies derive sequentially from a differentiation pathway that is regulated by activation-dependent post-thymic differentiation.
    J Immunol. 1988 Sep 1;141(5):1464-70 PMID: 2970504
  8. Differential activation requirements for virgin and memory T cells.
    J Immunol. 1988 Nov 15;141(10):3249-57 PMID: 2972769
  9. Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.
    Eur J Immunol. 1988 Nov;18(11):1653-61 PMID: 2974420
  10. Monoclonal antibody to a triggering structure expressed on rat natural killer cells and adherent lymphokine-activated killer cells.
    J Exp Med. 1989 Apr 1;169(4):1373-89 PMID: 2466943
  11. Peripheral T lymphocytes: expansion potential and homeostatic regulation of pool sizes and CD4/CD8 ratios in vivo.
    Eur J Immunol. 1989 May;19(5):905-11 PMID: 2500349
  12. Human naive and memory T cells: reinterpretation of helper-inducer and suppressor-inducer subsets.
    Immunol Today. 1988 Jul-Aug;9(7-8):195-9 PMID: 2978373
  13. Phenotypic and functional heterogeneity of CD4+ T cells.
    Immunol Today. 1988 Sep;9(9):274-7 PMID: 2908230
  14. Fidelity of the repertoire in T cell reconstituted athymic nude rats. Preservation of a deficit in alloresponsiveness over one year.
    J Exp Med. 1989 Sep 1;170(3):691-702 PMID: 2788706
  15. Subsets of thymopoietic rat thymocytes defined by expression of the CD2 antigen and the MRC OX-22 determinant of the leukocyte-common antigen CD45.
    Eur J Immunol. 1989 Dec;19(12):2289-95 PMID: 2481590
  16. The origin of T cells in permanently reconstituted old athymic nude rats. Analysis using chromosome or allotype markers.
    Immunology. 1989 Dec;68(4):547-56 PMID: 2575076
  17. Kinetics of mature T-cell development in the thymus.
    Proc Natl Acad Sci U S A. 1990 Apr;87(7):2579-82 PMID: 2138780
  18. Allograft rejection in athymic nude rats by transferred T cell subsets. II. The response of naive CD4+ and CD8+ thoracic duct lymphocytes to an isolated MHC class I disparity.
    Transplantation. 1990 Oct;50(4):690-6 PMID: 1977220
  19. Interconversion of CD45R subsets of CD4 T cells in vivo.
    Nature. 1990 Nov 8;348(6297):163-6 PMID: 1978253
  20. CD3+ T cells in severe combined immune deficiency (scid) mice. I. Transferred purified CD4+ T cells, but not CD8+ T cells are engrafted in the spleen of congenic scid mice.
    Eur J Immunol. 1991 Feb;21(2):523-33 PMID: 1671840
  21. Peripheral selection of the T cell repertoire.
    Science. 1991 Mar 8;251(4998):1225-8 PMID: 1900951
  22. Analysis of recent thymic emigrants with subset- and maturity-related markers.
    Int Immunol. 1990;2(5):419-25 PMID: 2085486
  23. CD45R CD4 T cell subset-reconstituted nude rats: subset-dependent survival of recipients and bi-directional isoform switching.
    Eur J Immunol. 1991 Apr;21(4):993-1000 PMID: 1673436
  24. Phenotypic and functional stages in the intrathymic development of alpha beta T cells.
    Immunol Today. 1991 Feb;12(2):65-70 PMID: 2059306
  25. T-cell receptor-bearing cells from athymic nude rats respond to alloantigen in vitro but are defective in vivo.
    Immunology. 1991 Jul;73(3):334-41 PMID: 1831792
  26. Normal development and function of CD8+ cells but markedly decreased helper cell activity in mice lacking CD4.
    Nature. 1991 Sep 12;353(6340):180-4 PMID: 1832488
  27. Post- thymic in vivo expansion of mature alpha beta T cells.
    Int Immunol. 1991 Nov;3(11):1077-80 PMID: 1836957
  28. Memory in helper T cells of minor histocompatibility antigens, revealed in vivo by alloimmunizations in combination with Thy-1 antigen.
    Eur J Immunol. 1992 Jan;22(1):115-22 PMID: 1346105
  29. Allograft rejection in CD4+ T cell-reconstituted athymic nude rats--the nonessential role of host-derived CD8+ cells.
    Transplantation. 1992 Feb;53(2):477-82 PMID: 1346733
  30. Function of CD4 T cell subsets in vivo: expression of CD45R isoforms.
    Semin Immunol. 1992 Feb;4(1):43-50 PMID: 1534263
  31. Functional maturation of recent thymic emigrants in the periphery: development of alloreactivity correlates with the cyclic expression of CD45RC isoforms.
    Eur J Immunol. 1992 Sep;22(9):2261-9 PMID: 1355430
  32. Differential thymus dependence of rat CD8 isoform expression.
    Eur J Immunol. 1992 Nov;22(11):2841-8 PMID: 1358623
  33. Lifespan of human lymphocyte subsets defined by CD45 isoforms.
    Nature. 1992 Nov 19;360(6401):264-5 PMID: 1436108
  34. Rapid re-expression of CD45RC on rat CD4 T cells in vitro correlates with a change in function.
    Eur J Immunol. 1993 Jan;23(1):103-9 PMID: 8093440
  35. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues.
    J Immunol. 1993 Mar 1;150(5):1670-9 PMID: 8094727
  36. Lymphocyte lifespans: homeostasis, selection and competition.
    Immunol Today. 1993 Jan;14(1):25-9 PMID: 8442858
  37. B-cell lifespans in the mouse--why to debate what?
    Immunol Today. 1993 Jan;14(1):40-1; discussion 41-3 PMID: 8442863
  38. The life span of naive alpha/beta T cells in secondary lymphoid organs.
    J Exp Med. 1993 Apr 1;177(4):891-6 PMID: 8459219
  39. Virus persistence in acutely infected immunocompetent mice by exhaustion of antiviral cytotoxic effector T cells.
    Nature. 1993 Apr 22;362(6422):758-61 PMID: 8469287
  40. CD4+ T cell-mediated rejection of major histocompatibility complex class I-disparate grafts: a role for alloantibody.
    Eur J Immunol. 1993 Sep;23(9):2078-84 PMID: 8103742
  41. Turnover of naive- and memory-phenotype T cells.
    J Exp Med. 1994 Apr 1;179(4):1127-35 PMID: 8145034
  42. Selective events in T cell development.
    Annu Rev Immunol. 1994;12:675-705 PMID: 8011294
  43. Membrane CD45R isoform exchange on CD4 T cells is rapid, frequent and dynamic in vivo.
    Eur J Immunol. 1994 Nov;24(11):2573-8 PMID: 7957551
  44. The lymphoreticular system in triggering virus plus self-specific cytotoxic T cells: evidence for T help.
    J Exp Med. 1978 Mar 1;147(3):897-911 PMID: 305460
  45. Studies on hyperlymphoid mice.
    Immunology. 1979 Sep;38(1):163-71 PMID: 315914
  46. Thymus cell migration. Quantitative aspects of cellular traffic from the thymus to the periphery in mice.
    Eur J Immunol. 1980 Mar;10(3):210-8 PMID: 7379836
  47. Phenotype of rat natural killer cells defined by monoclonal antibodies marking rat lymphocyte subsets.
    Immunology. 1982 Jan;45(1):97-103 PMID: 7056570
  48. The functional capabilities of cells leaving the thymus.
    J Immunol. 1984 Jan;132(1):25-30 PMID: 6197445
  49. T cell repopulation from functionally restricted splenic progenitors: 10,000-fold expansion documented by using limiting dilution analyses.
    J Immunol. 1984 Dec;133(6):2925-32 PMID: 6238091
  50. Early T lymphocytes. Differentiation in vivo of adult intrathymic precursor cells.
    J Exp Med. 1985 Sep 1;162(3):802-22 PMID: 2863322
  51. Postthymic T-cell development.
    Immunol Rev. 1986 Jun;91:159-94 PMID: 3525393
Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1995-04-00
Pages
514-20
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1415168
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2