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PMID: 7799923 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA synthesis generally initiates outside the simian virus 40 core origin in vitro.

Molecular and cellular biology ·Vol. 15 ·No. 1 ·1995-01-00 ·Pages 173-8

Bullock PA, Denis D

Abstract

The nucleotide positions at which DNA synthesis initiates in vitro, in the vicinity of the simian virus 40 origin, have been determined. Start sites for DNA synthesis are greatly suppressed over the simian virus 40 core origin. Relatively weak start sites are detected over the 21-bp repeats and T-antigen-binding site I; distal to these regions, stronger start sites are detected. Thus, studies using a model system for eukaryotic DNA replication indicate that DNA synthesis events initiate, in general, outside the core origin.

MeSH Terms
Base Sequence Cell-Free System DNA Replication DNA, Viral/genetics In Vitro Techniques Molecular Sequence Data RNA, Viral/metabolism Regulatory Sequences, Nucleic Acid Replicon Simian virus 40/genetics Virus Replication
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bullock P A
Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111.
Denis D
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47 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.1.173
Published
1995-01-00
Pages
173-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231928
Subset
IM
Grants
NIAID NIH HHS · R01 AI30032 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.1.173 PMC Full Text View journal details
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