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PMID: 7799951 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Spontaneous in vitro immortalization of breast epithelial cells from a patient with Li-Fraumeni syndrome.

Molecular and cellular biology ·Vol. 15 ·No. 1 ·1995-01-00 ·Pages 425-32

Shay JW, Tomlinson G, Piatyszek MA, Gollahon LS

Abstract

Individuals with germ line mutations in the p53 gene, such as Li-Fraumeni syndrome (LFS), have an increased occurrence of many types of cancer, including an unusually high incidence of breast cancer. This report documents that normal breast epithelial cells obtained from a patient with LFS (with a mutation at codon 133 of the p53 gene) spontaneously immortalized in cell culture while the breast stromal fibroblasts from this same patient did not. Spontaneous immortalization of human cells in vitro is an extremely rare event. This is the first documented case of the spontaneous immortalization of breast epithelial cells from a patient with LFS in culture. LFS patient breast stromal fibroblasts infected with a retroviral vector containing human papillomavirus type 16 E7 alone were able to immortalize, whereas stromal cells obtained from patients with wild-type p53, similarly infected with human papillomavirus type 16 E7, did not. The present results indicate a protective role of normal pRb-like functions in breast stromal fibroblasts but not in breast epithelial cells and reinforces an important role of wild-type p53 in the regulation of the normal growth and development of breast epithelial tissue.

MeSH Terms
Adult Base Sequence Breast/cytology Breast Neoplasms/pathology Cells, Cultured Cellular Senescence DNA Nucleotidylexotransferase/metabolism Epithelial Cells Female Genes, p53 Humans In Vitro Techniques Karyotyping Li-Fraumeni Syndrome/pathology Molecular Sequence Data Pedigree
Chemicals
DNA Nucleotidylexotransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shay J W
Department of Cell Biology and Neurosciences, University of Texas Southwestern Medical Center at Dallas 75235-9039.
Tomlinson G
Piatyszek M A
Gollahon L S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.1.425
Published
1995-01-00
Pages
425-32
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231985
Subset
IM
Grants
NCI NIH HHS · CA50195 · United States
NCI NIH HHS · CA64871 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.1.425 PMC Full Text View journal details
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