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PMID: 780345 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells.

Journal of bacteriology ·Vol. 126 ·No. 3 ·1976-06-00 ·Pages 1250-60

Beck BD, Park JT

Abstract

The specific activities of three murein hydrolases, carboxypeptidase I, carboxypeptidase II, and amidase were studied with respect to cell division in toluene-treated cells of Escherichia coli K-12. Carboxypeptidase I and amidase activities were constant throughout the division cycle in cells of D11/lac+pro+. Detectable carboxypeptidase II activity varied and was highest at the time of division by a factor of three. Carboxypeptidase II specific activity was also correlated with cell division in BUG 6, a temperature-sensitive mutant (J.N Reeve, D.J. Groves, and D.J. Clark, 1970). Fifteen minutes after shifting BUG 6 from 42 C (nondividing conditions) to 32 C (dividing conditions), there was a rapid resumption of cell division, accompanied by a 10-fold increase in the specific activity of carboxypeptidase II. These results demonstrate a correlation between detectable carboxypeptidase II activity and cell division as reflected by activity in toluene-treated cells. The subcellular location of carboxypeptidase II, a soluble enzyme was found to be periplasmic since it was released by tris(hydroxymethyl)-aminomethane-ethylenediaminetetraacetate treatment and osmotic shock, two methods known to release periplasmic enzymes.

MeSH Terms
Amidohydrolases/metabolism Carboxypeptidases/metabolism Cell Division Cell-Free System Cytoplasm/enzymology Escherichia coli/drug effects,enzymology,growth & development Galactosidases/metabolism Hydrolysis Mutation Peptidoglycan/metabolism Subcellular Fractions/enzymology Temperature Toluene/pharmacology
Chemicals
Peptidoglycan Toluene Galactosidases Carboxypeptidases Amidohydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Beck B D
Park J T
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48 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.3.1250-1260.1976
Published
1976-06-00
Pages
1250-60
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233150
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.3.1250-1260.1976 PMC Full Text View journal details
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